International Journal of Molecular Evolution and Biodiversity, 2025, Vol.15, No.2, 99-110 http://ecoevopublisher.com/index.php/ijmeb 99 Research Report Open Access Study on the Genetic Diversity and Superior Gene Screening of Lindera aggregataGermplasm Resources in Qingchuan County Jianli Zhong Hainan Institute of Biotechnology, Haikou, 570206, Hainan, China Corresponding email: jianli.zhong@hitar.org International Journal of Molecular Evolution and Biodiversity, 2025, Vol.15, No.2 doi: 10.5376/ijmeb.2025.15.0009 Received: 12 Feb., 2025 Accepted: 18 Mar., 2025 Published: 02 Apr., 2025 Copyright © 2025 Zhong, This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Zhong J.L., 2025, Study on the genetic diversity and superior gene screening of Lindera aggregata germplasm resources in Qingchuan county, International Journal of Molecular Evolution and Biodiversity, 15(2): 99-110 (doi: 10.5376/ijmeb.2025.15.0009) Abstract This study analyzed the genetic diversity characteristics and ecological adaptability mechanisms of the germplasm resources of Lindera aggregata in Qingchuan County, and revealed the influence of different ecological environments on the genetic variation and trait expression of Lindera aggregata. The study identified multiple functional genes closely related to key medicinal components (volatile oils, total phenols, total flavonoids) and stress resistance (drought resistance, cold resistance, and salt resistance), some of which showed significant expression differences under specific environmental stresses. The study found that the germplasm resources of Lindera aggregata in Qingchuan County have rich genetic diversity, and their genetic structure characteristics are significantly correlated with the ecological environment. Based on gene function annotation, a number of core genes regulating secondary metabolic synthesis and environmental adaptability were successfully excavated. Combined with the comprehensive evaluation of multiple traits, several excellent germplasms with outstanding comprehensive traits were screened, and regional adaptive cultivation strategies were constructed accordingly. This study not only provides a necessary meal card for the protection of Lindera aggregata germplasm resources, but also lays a theoretical foundation for molecular breeding practice. The research results will effectively promote the quality improvement and yield stability of Lindera aggregata, and have important practical value for promoting the sustainable development of the Chinese medicinal materials industry. Keywords Lindera aggregata; Germplasm resources; Genetic diversity; Superior gene screening; Molecular breeding 1 Introduction As an important representative of the medicinal plants of the Lauraceae family, Lindera aggregata occupies a special position in the traditional Chinese medicine system due to its unique chemical composition and significant clinical efficacy. Studies have shown that the active ingredients contained in the roots and leaves of this plant can effectively regulate multiple metabolic pathways and have significant therapeutic effects on diseases such as chronic kidney disease. From ancient classic prescriptions such as "Suquan Pills" to modern medical practice, the medicinal value of Lindera aggregata has been continuously verified (Cai et al., 2020). It is worth noting that the unique Lindera aggregata germplasm resources in Qingchuan County have become key genetic materials for improving the quality of medicinal materials and promoting industrial development due to their excellent genetic characteristics (Shi et al., 2024a; Shi et al., 2024b). In-depth analysis of the genetic diversity of Lindera aggregata is of great significance for the protection and development and utilization of its resources. Molecular marker analysis based on chloroplast and nuclear DNA can not only clarify the evolutionary process of species, but also provide key evidence for the study of population genetic structure (Ye and Li, 2019; Ye and Li, 2021). More importantly, screening for excellent functional genes can directly improve the medicinal quality, yield potential and stress resistance of Linderae obesa, which has a dual role in enhancing its environmental adaptability and medicinal value (Xiong et al., 2020; Shi et al., 2024a). This study intends to use the germplasm of Lindera aggregata in Qingchuan County as the research core, use modern molecular biological methods to systematically analyze its genetic diversity structure, and screen functional genes closely related to pharmacodynamic properties and ecological adaptability. This study will provide theoretical support and technical reserves for the protection, utilization and resource innovation of this special medicinal plant.
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