Triticeae Genomics and Genetics, 2025, Vol.16, No.6, 269-277 http://cropscipublisher.com/index.php/tgg 274 that many people are concerned about. From the experimental results, it can be seen that the expression range of this type of promoter is indeed relatively limited, and the risk of off-target is relatively small. But things don't end there. Even if the effect of genetically modified wheat on rhizosphere soil microorganisms is not obvious, the long-term changes of the ecosystem still need to be observed (Lebedev et al., 2022). And will genes be transmitted to wild relatives through pollen? Or will it have subtle but cumulative effects on non-target organisms? These issues cannot be simply attributed to "low risk" and thus be approved. Continuous ecological monitoring and management remain necessary. Figure 2 Morphologies of root tips and root cross sections of wild-type and different taare1 mutant lines (Adopted from Zhang et al., 2021) 7.2 Positive impact of nitrogen fertilizer reduction on environmental pollution control It is almost common sense in agriculture that the more nitrogen fertilizer is input, the higher the possibility of
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