Triticeae Genomics and Genetics, 2025, Vol.16, No.3, 138-147 http://cropscipublisher.com/index.php/tgg 140 gradually decline. The aleurone layer, starch endosperm, transferred cells and other regions each exhibit different expression profiles. Currently, 10 types of cells can be distinguished and 192 marker genes have been identified. Transcription factors like TaABI3-B1 are particularly active only in the embryo and its adjacent endosperm. They regulate not only the embryo size but also influence the final formation of the entire grain. These findings once again demonstrate the significance of spatial dimension expression regulation in terms of yield and quality (Figure 1) (Li et al., 2025). Figure 1 Spatially resolved transcriptome analysis of wheat grains. A A workflow for sampling and sequencing of wheat grains on a BMKMANU S1000. B Developing grains at dap 4, dap 8, and dap 12. C Spatial visualization of the unbiased spot clustering for dap 4, dap 8, and dap 12 sections. Merged bright field images and spatial clusters of the other three sections. The tissue/cell-type identity of each cluster was assigned based on the location of each cluster. D Heatmap and spatial distribution map of total expression counts in the spot of each sample. E UMAP of spatial spots from dap 4, dap 8, and dap 12 wheat sections. Dots correspond to individual spots on the BMKMANU S1000 chip; colors indicate cell type annotation for each spot (Adopted from Li et al., 2025)
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