Rice Genomics and Genetics 2025, Vol.16, No.4, 199-210 http://cropscipublisher.com/index.php/rgg 201 Figure 1 Schematic overview of grain development in rice (Adopted from Liu et al., 2025) Image caption: (A) The relative volume of caryopsis within the grain and morphological event is presented throughout the developmental process of rice grain. Total starch and protein content in grain (relative to maximum volume) is shown in parallel with the time course (days after pollination (DAP)). (B) Timeline diagram of developmental differences and hormone level changes between superior and inferior grains. The solid lines represent superior spikelet grains (SS), and the dashed lines represent inferior spikelet grains (IS), illustrating the developmental differences and changes in hormone levels over time (Adopted from Liu et al., 2025) However, the initial steps of doing scRNA-seq on plants are actually not simple. For example, to isolate living single cells or cell nuclei from tissues, this step often requires enzymes for processing because the cell walls of
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