Rice Genomics and Genetics 2024, Vol.15, No.3, 121-131 http://cropscipublisher.com/index.php/rgg 124 Figure 2 Binding of MOC3to FON1promoter activates FON1expression (Adopted from Shao et al., 2019) Image caption: (A) MOC3 binding profile on the FON1 promoter; The black line represents the ChIP-seq peak, and the red line indicates probe 1 used in the EMSA experiment; Sites P1 to P6 were used for the ChIP-qPCR experiment; (B) ChIP-qPCR analysis validating MOC3 binding sites on the FON1 promoter using MOC3 polyclonal antibodies in the 0.3 cm shoot bases of wild-type Nipponbare seedlings; Fold enrichment values were normalized against the Ubiquitin promoter; Values are means±SEM (n=3); **P<0.01; ns, not significant; (C) EMSA experiment showing that MOC3 can directly bind to probe 1; Competition was performed with 20-fold and 200-fold excess unlabeled probes; (D) FON1 expression levels in wild type (WT) and moc3-2; Values are means±SEM (n=3); **P<0.01; Ten-day-old seedlings were used for qRT-PCR; (E) Transient transcriptional activity assay showing that MOC3 activates the expression of FON1 in rice protoplasts; The left panel shows the constructs used in the transient expression assays; Values are means±SD (n=3); **P<0.01 (Adapted from Shao et al., 2019) MOC1 also plays a significant role in optimizing resource allocation within the rice plant. By balancing the growth of tillers and the development of the root system, MOC1 ensures that the plant can efficiently utilize available resources, such as water and nutrients. This optimization is crucial for sustaining high yields, especially under varying environmental conditions. Efficient resource allocation driven by MOC1 helps maintain a stable yield and improves the plant's overall fitness and resilience.
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