Rice Genomics and Genetics 2024, Vol.15, No.2, 69-79 http://cropscipublisher.com/index.php/rgg 72 Figure 1 Molecular mechanism of Pijx gene regulating rice blast resistance (Xiao et al., 2023) Molecular markers and mapping also help scientists gain a deeper understanding of the genetic mechanism of rice blast resistance genes. By locating these genes, scientists can study information such as their location in the rice genome and their interactions with other genes, which provides the basis for in-depth exploration of the rice blast resistance mechanism. For example, by locating rice blast resistance genes, scientists found that some resistance genes may be closely related to the plant's immune system, thus providing clues to further analyze the disease resistance mechanism of rice (Xiao et al., 2023). Advances in molecular marker technology have also provided more possibilities for the discovery of rice blast resistance genes. With the development of high-throughput sequencing technology, scientists can analyze the rice genome more quickly and comprehensively, making it easier to discover genes related to rice blast resistance. For example, through whole-genome sequencing technology, scientists have successfully discovered some new rice blast resistance genes. The existence of these genes provides new candidate genes for breeding more disease-resistant rice varieties in the future. Molecular marker technology can also help analyze the diversity of rice blast resistance genes. Different rice varieties may carry different resistance genes, and the differences in these genes may be related to the geographical distribution of the varieties, ecological environment and other factors. By molecularly marking and locating the resistance genes of different varieties, scientists can map the distribution of rice blast resistance genes, reveal their diversity and distribution patterns, and provide a scientific basis for the rational selection of rice varieties suitable for different regions. 3 Molecular Identification of Rice Blast Resistance Genes 3.1 Application of genomics technology The application of genomics technology in the agricultural field, especially whole-genome sequencing technology, provides a powerful tool for the discovery of resistance genes. Whole-genome sequencing is a high-throughput genetic analysis method that can quickly and accurately reveal the entire genome information of an organism. In the study of rice resistance genes, the application of whole-genome sequencing technology has made remarkable achievements. An important application of whole-genome sequencing technology is in the discovery of resistance genes. By performing whole-genome sequencing of resistant varieties against rice blast, scientists are able to gain a comprehensive understanding of the composition of the rice genome and quickly identify genes associated with resistance. As mentioned before, by conducting whole-genome sequencing of a highly resistant rice variety,
RkJQdWJsaXNoZXIy MjQ4ODYzNA==