Rice Genomics and Genetics 2024, Vol.15, No.1, 28-35 http://cropscipublisher.com/index.php/rgg 32 comparison of the panicles of ZH11 and Osjar1 mutants at 11:30 AM in June 2022 in Guangzhou, with a scale of 1 cm. c) Recorded the number of florets opened per panicle at different times of the day for ZH11 and Osjar1 mutants, with the mean values presented as standard error (SEM) (n=10 panicles). d) and e) Measured the JA-Ile content in the lodicules of ZH11, Osjar1 mutants, and Osmyb8ZH mutants at 10:00 AM and 9:00 AM, respectively, with values presented as standard error (SEM) (n=3 biological replicates). f) Schematic diagram showing the vector structure used to construct OsJAR1com materials, where pOsMYB8TF represents the promoter amplified from TFB. g) Showed the relative expression levels of OsJAR1 in the lodicules of ZH11, OsJAR1com, and Osmyb8ZH, with values presented as standard error (SEM) (n=3 biological replicates). h) and j) Showed a comparison of the panicles of ZH11, OsJAR1com, and Osmyb8ZH as well as ZH11, OsMYB8TF/ZH11, OsMYB8TF/Osjar1, and Osjar1 at 11:30 AM and 12:00 PM in June and October 2022 in Guangzhou, respectively, with a scale of 1 cm. i) and k) Recorded the number of florets opened at different times of the day for ZH11, OsJAR1com, and Osmyb8ZH as well as ZH11, OsMYB8TF/ZH11, OsMYB8TF/Osjar1, and Osjar1, with mean values presented as standard error (SEM) (n=10 panicles). Figure 5 OsJAR1 influences JA-Ile content in lodicule to regulate rice DFOT Therefore, the results indicate that OsJAR1 not only regulates rice DFOT by affecting the content of JA-Ile but also involves other complex biological processes. The data provided in the figures highlight the important application of gene editing technology in functional gene research and the potential for regulating flowering time in rice breeding. Figure 6 revealed how natural variation in the OsMYB8 promoter causes differences in the diurnal floret opening time (DFOT) between indica and japonica rice. a) Single nucleotide polymorphism analysis of the OsMYB8 promoter was conducted in 3513 rice germplasm resources, showing the variation in the 2 kb promoter region. b) Displayed the distribution frequency of three OsMYB8 haplotypes in different rice germplasms across Asia, with haplotype 1 (Hap1) being the most common. c) Analyzed the nucleotide diversity (π value) around the 100 kb genomic region surrounding the OsMYB8 gene, comparing the differences among indica (Ind), temperate
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