Rice Genomics and Genetics 2024, Vol.15, No.1, 12-18 http://cropscipublisher.com/index.php/rgg 12 Research Article Open Access Development of CRISPR-Cas9 Multiple Editing System for Genetic Improvement of Rice YuWang Biotechnology Research Center, Cuixi Academy of Biotechnology, Zhuji, 311800, Zhejiang, China Corresponding author email: yuwang@hotmail.com Rice Genomics and Genetics, 2024, Vol.15, No.1 doi: 10.5376/rgg.2024.15.0002 Received: 03 Dec., 2023 Accepted: 07 Jan., 2024 Published: 26 Jan., 2024 Copyright © 2024 Wang, This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Wang Y., 2024, Development of Crispr-Cas9 multiple editing system for genetic improvement of rice, Rice Genomics and Genetics, 15(1): 12-18 (doi: 10.5376/rgg.2024.15.0002) Abstract The CRISPR-Cas9 multiple editing system has become an important tool in the field of rice genetic improvement. This review aims to outline the principles and applications of the system, emphasizing its cutting-edge position in rice breeding. The advantage of a multiple editing system is that it can simultaneously edit multiple loci to achieve precise improvement of rice yield, resistance, and quality traits. This review also discusses in detail the working principle, development process, and widespread application of multiple editing systems, briefly introduces CRISPR-Cas9 technology, explains how multiple editing systems can achieve efficient multi gene editing, and delves into the specific applications of multiple editing systems in rice genetic improvement, including increasing yield, increasing resistance, and improving quality. These applications have enriched the genetic resources of rice and provided new avenues for food security and sustainable agricultural development. Keywords CRISPR Cas9; Multiple editing system; Rice breeding; Increased production; Enhanced disease resistance 1 Introduction Rice (Oryza sativa) is one of the most important food crops in the world, providing a major source of food for many people worldwide. With the growth of global population and the threat of climate change, ensuring the improvement of rice yield, quality, and resistance has become particularly urgent. In the past few decades, traditional breeding methods have made significant progress, but their speed and efficiency are still limited. Fortunately, breakthroughs in modern molecular biology and gene editing technology have opened up new avenues for genetic improvement in rice. CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR associated protein 9) is a revolutionary gene editing technique that allows scientists to modify genes at specific locations in rice and other plants. However, the initial CRISPR-Cas9 technology had some limitations, one of which was that only one gene could be edited. Over time, researchers have developed the CRISPR-Cas9 multi editing system, and the introduction of this technology has changed the game rules of rice genetic improvement (Romero et al., 2019). The purpose of this review is to comprehensively explore the application of CRISPR-Cas9 multiple editing system in rice genetic improvement. This review will focus on introducing the working principle, development history, and cutting-edge position of multiple editing system in rice breeding. The uniqueness of a multi editing system lies in its ability to simultaneously edit multiple gene loci, providing new opportunities for precise shaping of rice quality and traits. This review briefly reviews the basic principles of CRISPR-Cas9 technology to ensure readers have a basic understanding of this technology, and delves into the construction and working methods of multiple editing systems, explaining how to achieve efficient multi gene editing. In addition, this review will also discuss in detail the specific applications of multiple editing systems in rice genetic improvement, including how to increase rice yield, increase resistance, and improve quality. These applications not only enrich the genetic resources of rice, but also provide new avenues for global food security and sustainable agricultural development.
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