Maize Genomics and Genetics 2025, Vol.16, No.6, 325-333 http://cropscipublisher.com/index.php/mgg 330 signs of chlorosis, wilting or even withering very early. If the antioxidant index, MDA content and enzyme activity parameters are superimposed, these physiological differences become even clearer (Figure 2) (Ji et al., 2025). Figure 2 Salt treatment phenotypic characterization of the ST and SS maize cultivars (Adopted from Ji et al., 2025) Image caption: (A) Phenotypes of the ST and SS seedings to salt stress. Plants were grown to the four-leaf stage in soil and then irrigated with saline water containing 300 mM NaCl for 0, 3, and 7 days. The scale bars are six cm. (B) DAB and NBT staining of the third leaves of ST and SS seedings treated with 300 mM NaCl for 0, 3 and 7 days. The scale bars are one cm. (C) The RWC, REL, H2O2 and MDA content, SOD and POD activities of ST and SS seedings treated with 300 mM NaCl for 0, 3 and 7. Data means ± SDs (n = 3). The symbols * and ** indicate significant differences between ST and SS seedings at P < 0.05 and P < 0.01, respectively (Adopted from Ji et al., 2025) 6.2 Methylation pattern variations and identification of salt tolerance-related loci DNA methylation is no longer a "new concept" in salt tolerance research, but its performance among different strains does have many aspects worth pondering. Especially at the CHH and CHG sites, the methylation reaction
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