Legume Genomics and Genetics 2025, Vol.16, No.5, 234-244 http://cropscipublisher.com/index.php/lgg 239 Figure 2 Domain compositions of adzuki bean DCL (A), AGO (B), and RDR (C) protein sequences. Domains are indicated as boxes in various colors. The diagrams were drawn to scale (Adopted from Li et al., 2023) 6.3 Identification of cold-tolerant germplasm and breeding strategies for resilient varieties Not every adzuki bean variety is equally resilient to cold weather. The screening results of a large number of germplasm resources show that the performance of different varieties varies greatly, whether in the germination stage or the seedling stage. For instance, among 384 resources, only 31 demonstrated relatively outstanding cold resistance during the germination stage, while several species like GI-34, JN95-0, and E0064 were equally stable during the seedling stage. These well-performing materials are of great significance to breeding projects that aim to ensure both yield and enhance cold resistance. In addition, some studies have also found that exogenous spraying of ABA can help crops maintain a stable state at low temperatures. Although it is not a fundamental solution, it can reduce some losses at critical points and can be regarded as a practical supplementary path (Xiang et al., 2024).
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