Legume Genomics and Genetics 2025, Vol.16, No.3, 135-142 http://cropscipublisher.com/index.php/lgg 141 8 Conclusion Scientists use the CRISPR activation system to control native flowering gene expression with its programmable activation mechanism. CRISPRa enables scientists to activate specific flowering regulators including FT and related genes which results in new methods for crop adaptation and yield optimization and complex flowering network research in mung bean and additional crops. CRISPRa technology provides researchers with multiple benefits because it enables exact gene expression management through reversible and multiplexable methods that protect cells from harm which makes it suitable for studying complex traits such as flowering. However, technical limitations remain. The delivery of CRISPRa components faces multiple obstacles which include inefficient delivery systems and target-specific activation variability and off-target effects and insufficient regulatory structures and public support. Additionally, most current applications and case studies are concentrated in model plants and major crops, with translational work in mung bean still in its early stages. The future of mung bean development will benefit from CRISPRa integration with sophisticated editing tools and multiple gene activation methods to create varieties that match specific flowering schedules and show improved tolerance to various environmental conditions. The development of better delivery systems and clearer regulations will make CRISPRa a fundamental technology for sustainable mung bean breeding which will enhance food security and climate resilience by enabling fast and adaptable crop improvement. Acknowledgments I sincerely thank Dr. Li for providing valuable comments and suggestions during the writing of this paper, which were instrumental in improving its quality. Conflict of Interest Disclosure The author affirms that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Chaudhary B., Singh P., Jatav A., Singh M., Verma V., Kumar A., and Kumar D., 2024, Effect of plant growth regulators on phenological study and growth analysis of mung bean (Vigna radiata L.) under central plain zone of Uttar Pradesh, International Journal of Research in Agronomy, 7(10): 5-9. https://doi.org/10.33545/2618060x.2024.v7.i10a.1686 Chiteri K., Rairdin A., Sandhu K., Redsun S., Farmer A., O’Rourke J., Cannon S., and Singh A., 2024, Combining GWAS and comparative genomics to fine map candidate genes for days to flowering in mung bean, BMC Genomics, 25: 270. https://doi.org/10.1186/s12864-024-10156-x Ding X., Yu L., Chen L., Li Y., Zhang J., Sheng H., Ren Z., Li Y., Yu X., Jin S., and Cao J., 2022, Recent progress and future prospect of CRISPR/Cas-derived transcription activation (CRISPRa) system in plants, Cells, 11(19): 3045. https://doi.org/10.3390/cells11193045 Ellis R., Lawn R., Summerfield R., Qi A., Roberts E., Chay P., Brouwer J., Rose J., Yeates S., and Sandover S., 1994, Towards the reliable prediction of time to flowering in six annual crops. IV. cultivated and wild mung bean, Experimental Agriculture, 30(1): 31-43. https://doi.org/10.1017/S0014479700023826 Gao Y., Zhang X., Wang X., Zhang Q., Tang H., Qiu T., Zhang H., Zhao B., Wang H., Liang X., and Guo Y., 2022, Exogenous DCPTA treatment increases mung bean yield by improving carbon metabolism pathway and up-regulating photosynthetic capacity and antioxidants, Frontiers in Plant Science, 13: 796694. https://doi.org/10.3389/fpls.2022.796694 Hodaei A., and Werbrouck S., 2023, Unlocking nature’s clock: CRISPR technology in flowering time engineering, Plants, 12(23): 4020. https://doi.org/10.3390/plants12234020 Huppertz M., S, L., Kachhap D., Dalai A., Yadav N., Baby D., Khan M., Bauer P., and Panigrahi K., 2023, Exploring the potential of mung bean: from domestication and traditional selection to modern genetic and genomic technologies in a changing world, Journal of Agriculture and Food Research, 14: 100786. https://doi.org/10.1016/j.jafr.2023.100786 Imrie B., and Lawn R., 1990, Time to flowering of mung bean (Vigna radiata) genotypes and their hybrids in response to photoperiod and temperature, Experimental Agriculture, 26(3): 307-318. https://doi.org/10.1017/S0014479700018470
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