Legume Genomics and Genetics 2025, Vol.16, No.3, 135-142 http://cropscipublisher.com/index.php/lgg 135 Research Insight Open Access CRISPR Activation System for Flowering Regulation in Mung Bean Dandan Huang Hainan Institute of Biotechnology, Haikou, 570206, Hainan, China Corresponding email: dandan.huang@hibio.org Legume Genomics and Genetics, 2025 Vol.16, No.3 doi: 10.5376/lgg.2025.16.0014 Received: 13 Apr., 2025 Accepted: 28 May, 2025 Published: 16 Jun., 2025 Copyright © 2025 Huang, This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Huang D.D., 2025, CRISPR activation system for flowering regulation in mung bean, Legume Genomics and Genetics, 16(3): 135-142 (doi: 10.5376/lgg.2025.16.0014) Abstract This study demonstrates that CRISPR activation systems show great promise for mung bean flowering time control because they allow for yield improvement and adaptation. The research combines existing data about candidate flowering gene identification and functional analysis of VrFT1 and VrFT2 with CRISPRa applications for photoperiodic regulation in mung bean. The development of genome characterization and gene expression profiling techniques enables researchers to activate specific genes which should result in early or delayed flowering and better environmental tolerance. CRISPRa integration with advanced editing tools and multiplex gene activation methods shows potential for sustainable mung bean breeding and climate-resistant crop development but faces ongoing technical and regulatory hurdles. Keywords CRISPRa; Mung bean; Flowering regulation; Gene activation; Crop improvement 1 Introduction The legume crop Vigna radiata or mung bean serves as a crucial plant species because it contains abundant protein and grows quickly while tolerating various agricultural environments. The crop maintains a vital position in food security and economic stability because it functions as the main food source and revenue generator for Asian smallholder farmers. The improvement of mung bean production stands as a vital necessity to fulfill nutritional requirements of expanding human numbers while maintaining environmentally friendly farming practices. The process of flowering determines both the quantity and quality of crops as well as their ability to thrive in different environments. The timing of flower production determines when plants will reproduce during optimal environmental periods which affects seed production and crop yield. The control of flowering in mung bean and other crops enables better climate resistance and allows farming in different areas. The development of new crop improvement techniques depends heavily on the regulation of flower development. Genome editing technology has evolved through CRISPR activation (CRISPRa) systems which enable scientists to manage gene expression with exactness while keeping DNA sequences intact. The CRISPRa system uses dCas9 which lacks catalytic activity to link with transcriptional activators for boosting the expression of natural genes that control flowering processes. The method provides a solution to traditional transgenic overexpression problems because it enables researchers to activate multiple genes at once through guide RNA design (Karlson et al., 2021; Ding et al., 2022). The dual functionality of CRISPRa systems enables them to study flowering time regulators in model plants and crops by performing both functional gene analysis and trait development (Lowder et al., 2017; Lee et al., 2019; Ding et al., 2022; Hodaei and Werbrouck, 2023). This study aims to synthesize current knowledge on the application of CRISPRa systems for flowering regulation in mung bean. The research aims to demonstrate mung bean value in economic and agricultural practices and to explain the importance of flower control for breeding improvements and to assess CRISPRa technology capabilities for mung bean and other legume species.
RkJQdWJsaXNoZXIy MjQ4ODYzNA==