Legume Genomics and Genetics 2025, Vol.16, No.2, 63-71 http://cropscipublisher.com/index.php/lgg 65 middle that require manual verification. For instance, for some gene loci with ambiguous boundaries, further positioning needs to be done in combination with genomic annotation information. Figure 1 Expression analysis of group-III WRKY genes in different tissues of C. arietinum. The samples of chickpea were collected from 4-week-old plants for the root, stem, and leaf tissues. Bloomed flowers were sampled at reproductive stage while pod cover and seeds were collected at the same time points. The mRNA level expression was analysed by qRT-PCR using chickpea β-tubulin gene as internal control. The relative mRNA level was calculated with respect to the pod cover. The results presented here were obtained from three biological replicates with three technical replicates each. The error bars represent ±SD of means (Adopted from Kumar et al., 2016)
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