Legume Genomics and Genetics 2025, Vol.16, No.1, 33-43 http://cropscipublisher.com/index.php/lgg 36 Figure 1 GmVTC2b improved soybean tolerance to high light stress. (A) Expression pattern analysis of the GmVTC2b protein under darkness, LWL (50 μmol/m2/s) and HWL (1 800 μmol/m2/s). Three-week-old proGmVTC2b:GmVTC2b-Flag transgenic soybean seedlings were placed under darkness to equilibrate for 2 days and then transferred to LWL (50 μmol/m2/s), HWL (1 800 μmol/m2/s) and constant darkness. (B) Expression analysis of the GmVTC2b protein in response to LBL (5 μmol/m2/s), LRL (5 μmol/m2/s), HBL (500 μmol/m2/s) and HRL (600 μmol/m2/s). (C) The total AsA content was measured in 3-week-old leaves of GmVTC2b-ox and WT soybean under LD (16 h light/8 h darkness) with LWL and HWL, respectively. (D, E) (C) Maximum efficiency of PSII photochemistry (Fv/Fm ratio) in GmVTC2b-ox and WT soybean under high light stress (1 800 μmol/m2/s) for 0, 0.5, 1, 2 and 6 h in vivo imaging, and (D) the data were counted at 0 or 6 h. (F) (a) DAB and (b) NBT staining of the leaves in GmVTC2b-ox and WT soybean after 6 h of high light stress. (G) The H2O2 content was measured in the leaves of GmVTC2b-ox and WT soybean after 6 h of high light stress. (H) Phenotypic observations and the total AsA content showed that GmPLP1 inhibited the function of GmVTC2b under high light stress. Overexpression of pCAMBIA3300 (EV1), pFGC5941 (EV2), pCAMBIA3300-GmPLP1-GFP (GmPLP1-ox) and pFGC5941-GmPLP1 (RNAi) in proGmVTC2b:GmVTC2b-Flag (GmVTC2b-ox) transgenic soybean leaves. (a) Maximum PSII photochemistry efficiency (Fv/Fm ratio) under high light stress (1 800 μmol/m2/s) for 0, 0.5, 1, 2 and 6 h was captured using in vivo imaging, and (b) the data were obtained at 0 or 6 h. (c) The total AsA content was determined after 6 h. Values represent the means of three biological replicates. Asterisks indicate a significant difference from the corresponding controls (Student's t-test: 0.01 < *P <0.05, **P < 0.01) (Adopted from Zhang et al., 2023)
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