Legume Genomics and Genetics 2024, Vol.15, No.6, 291-302 http://cropscipublisher.com/index.php/lgg 295 Figure 2 Positions of the QTLs for drought tolerance on seven chromosomes (Ch) (Adopted from Dhungana et al., 2021) 5 Marker Development for Drought Tolerance in Soybean 5.1 Strategies for developing molecular markers linked to drought tolerance traits Developing molecular markers linked to drought tolerance traits in soybean involves several strategies. One primary approach is the identification and mapping of Quantitative Trait Loci (QTLs) associated with drought tolerance. This can be achieved through the use of Recombinant Inbred Line (RIL) populations derived from crosses between drought-tolerant and drought-sensitive cultivars. For instance, studies have identified multiple QTLs related to drought tolerance traits such as plant height and seed weight per plant using high-density genetic maps constructed with specific locus amplified fragment sequencing (SLAF-Seq) technology (Ren et al., 2020). Additionally, the use of Single Nucleotide Polymorphism (SNP) markers has been instrumental in constructing genetic maps and identifying QTLs associated with drought tolerance (Dhungana et al., 2021; Ouyang et al., 2022). Another strategy involves the use of Genome-Wide Association Studies (GWAS) to identify loci associated with drought tolerance. This method leverages the genetic diversity present in large germplasm collections to uncover significant associations between genetic markers and drought tolerance traits (Wang et al., 2020). Furthermore,
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