Legume Genomics and Genetics 2024, Vol.15, No.4, 199-209 http://cropscipublisher.com/index.php/lgg 201 CRISPR/Cas9-mediated gene editing has been utilized to enhance drought tolerance in soybeans by targeting and modifying drought-responsive genes. For instance, specific genes involved in water use efficiency and stress response pathways have been edited to improve the plant's ability to withstand drought conditions. These advancements demonstrate the potential of CRISPR/Cas9 in developing soybean varieties with enhanced drought tolerance, contributing to sustainable agricultural practices (Niazian et al., 2022). Figure 1 Base editing of GmFT2aandGmFT4 in soybean (Adopted from Cai et al., 2020) Image caption: (a) Gene structures of GmFT2a and GmFT4 with target sites for base editing. Black stripe, exon. Black line, intron. Grey stripe, untranslated regions. Nucleotides in blue represent the target sequences. Nucleotides in red represent the PAM (protospacer adjacent motif). (b, c) Sequences and peaks of representative mutation types of base editing of GmFT2a and GmFT4 in the T0 lines, respectively. The red arrowheads and underlines indicate the positions of these base editing mutations. (d) Base editing mutation types of GmFT2a in the T1 generation. (e) Sequence and peak of the homozygous ft2a mutant with C to G change. (f) and (g) Flowering time of WT, T2-ft2a-C7G-BE plants andft2a-+1A-Cas9 plants under SD conditions. Red box, magnified view. n, exact numbers of individual plants identified. **, P< 0.01. DAE, days after emergence. The flowering time is shown as the mean values ± standard deviation. (h) and (i) Flowering time of WT, T2-ft2a-C7G-BE plants and ft2a-+1A-Cas9 plants under LD conditions. Red box, magnified view. n, exact numbers of individual plants identified. **, P< 0.01. DAE, days after emergence. The flowering time is shown as the mean values ± standard deviation (Adopted from Cai et al., 2020)
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