Field Crop 2025, Vol.8, No.2, 72-81 http://cropscipublisher.com/index.php/fc 79 a promising prospect, it still has to go through several levels to promote it. In addition to optimizing editing efficiency, it is also necessary to consider the different regulatory policies of various countries and whether consumers can accept it. However, as the research deepens, I believe that these problems can be gradually solved. After all, compared with traditional breeding, CRISPR's advantages are still very obvious, especially in the precise regulation of fatty acid composition. 7 Concluding Remarks CRISPR technology is changing the way we improve the fatty acid composition of rapeseed. Recent studies have shown that by precisely editing specific genes, such as BnFAD2 and BnFAE1, oleic acid content can indeed be significantly improved (Shi et al., 2022). However, there are always some unexpected situations in actual operation. Sometimes the editing efficiency cannot be improved, which may be related to the genetic background of different rapeseed varieties. Now more and more laboratories are beginning to try to combine CRISPR and metabolomics (Karunarathna et al., 2020). This will not only provide a more comprehensive understanding of the fatty acid synthesis pathway, but also discover some unexpected regulatory nodes. Of course, this method requires more resources, which not every research team can afford. When it comes to practical applications, things are much more complicated. In addition to the optimization of the technology itself, we must also consider the differences in regulatory policies in different countries and the acceptance of consumers for gene-edited foods. But it is undeniable that compared with traditional breeding methods, CRISPR does have obvious advantages in precisely regulating fatty acid composition. If these practical problems can be solved in the future, the application prospects of this technology in rapeseed improvement should be more and more broad. There has been a new breakthrough in rapeseed breeding recently. By using CRISPR/Cas9 technology to tinker with genes that control fatty acid synthesis, we have indeed produced new varieties with better oil quality. However, there are many problems in actual operation, especially for polyploid crops such as rapeseed, which have too many gene copies and are particularly difficult to edit. The effect of increasing oleic acid content is quite obvious. Not only has the nutritional value increased, but the stability of the oil has also improved a lot. This is good news for the biofuel industry, but how much change it can bring depends on subsequent applications (Liu et al., 2022a). Interestingly, this editing method may also work on other polyploid crops, such as wheat and cotton. When it comes to improving oil quality, it is not just rapeseed. Oil crops such as soybeans and sunflowers should be able to learn from this method (Huang et al., 2020). Of course, each crop has its own characteristics and cannot be copied completely. The problem now is that although the effect is good in the laboratory, it still has to go through several hurdles to promote it on a large scale, including practical issues such as regulatory approval and farmer acceptance. CRISPR/Cas9 is a very useful tool for plant gene editing. Its most powerful feature is that it can accurately locate the gene to be modified, and its efficiency is much higher than the old method. In our experiment, we found that it worked well to adjust the oleic acid content of rapeseed, but there were still some minor problems in actual operation. Polyploid crops like rapeseed have a large number of gene copies, and it was almost impossible to edit multiple copies at the same time. But using CRISPR/Cas9 is much more convenient, although the success rate is not 100% (Zhang et al., 2019). When it comes to improving oil quality, increasing oleic acid content is indeed a breakthrough, but the specific improvement depends on subsequent stability tests (Liu et al., 2022a). This technology is developing very fast, and it is estimated that it will be able to show its prowess in rapeseed breeding in a short time. However, to truly promote it, it may be necessary to optimize the editing efficiency. After all, agricultural applications have to consider the cost issue, and farmers will not pay for flashy technology. Acknowledgments I am grateful to Mrs. Yang for critically reading the manuscript and providing valuable feedback that improved the clarity of the text. I express my heartfelt gratitude to the two anonymous reviewers for their valuable comments on the manuscript. Conflict of Interest Disclosure The author affirms that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest.
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