Field Crop 2024, Vol.7, No.6, 287-297 http://cropscipublisher.com/index.php/fc 290 stress (Li et al., 2022). By understanding these physiological mechanisms, researchers can develop strategies to improve the stress resistance of kiwifruit, ensuring better crop yield and quality under adverse environmental conditions. Figure 2 The AceGGP3 expression is induced by cold stress (Adopted from Liu et al., 2023) Image caption: (A-B) Gene ontology (GO) analysis of significantly up-regulated (A) and down-regulated (B) genes in kiwifruit calli at RT (room temperature) and COLD (4 °C) for 6 h. (C) Expression profiles of 21 genes of L-galactose pathway and 3 transcription factors in kiwifruit under RT and COLD. Log-transformed expression values range from 0 to 1. (D) RT-qPCR analysis of AceGGP3 expression of kiwifruit (A. eriantha) calli treated at 4 °C for 0 h, 2 h, 6 h, 12 h, 1 d, and 3 d. E) Relative AsA content of kiwifruit calli in (D). AsA content of calli in 0 h was taken as 5 for normalization. F) Relative GUS activity and histochemical staining of the AceGGP3 promoter expression construct AceGGP3:: GUS in transgenic kiwifruit calli at 25 °C (control) and 4 °C (cold stress) for 9 h. Error bars denote the standard deviation (± SD), n= 3. Significant differences were detected byt-test (*P<0.05; **P<0.01; ***P < 0.001) (Adopted from Liu et al., 2023)
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