Field Crop 2024, Vol.7, No.3, 134-144 http://cropscipublisher.com/index.php/fc 140 Additionally, there is a need to explore the genetic basis of resistance in cotton cultivars. The integration of advanced breeding techniques, including genomic selection and gene editing, can expedite the development of resistant varieties (Egan and Stiller, 2022). Research should also focus on optimizing agronomic practices, such as planting density and sowing dates, to mitigate the effects of seedling diseases and enhance overall crop productivity (Khan et al., 2017). Figure 2 Virulence tests of DVdRGS1; a: Disease symptoms of cotton plants (Junmian 1) infected with DVdRGS1 compared with wild-type Vd8 and VdRGS1-com strains. Photographs were taken at 11, 15 and 20 days postinoculation; b: Vascular discoloration of cotton plants inoculated with indicated strains. Plants were inoculated with Verticillium dahliae spore suspensions (107 conidia/mL) for 15 days, and then, the stems were cut and photographed by stereoscope (Olympus MVX10, Tokyo, Japan); c: The percentage of diseased leaves inoculated with DVdRGS1 compared with wild-type Vd8 and VdRGS1-com strains; d: Disease index (DI) of cotton plants inoculated the wild-type Vd8, DVdRGS1 and VdRGS1-com at 20 dpi. These experiments were repeated for three times using at least 40 seedlings per treatment, Error bars show the standard deviation of three biological replicates, Asterisks indicate statistically signi fi cant differences in the percentage of diseased leaves and the DI of plants treated with DVdRGS1 and VdRGS1-com mutants and the wildtype Vd8 strain, as determined by Student’s t-tests (*P<0.05, **P<0.01) (Adopted from Xu et al., 2018) Image caption: This figure shows that the severity of plant diseases can be attenuated by inhibiting the expression of the VdRGS1 gene. These experimental results show the importance of the VdRGS1 gene in plant disease response, and reducing the expression level can effectively reduce the occurrence of disease. This provides important reference value for further research and application (Adopted from Xu et al., 2018)
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