Cotton Genomics and Genetics 2025, Vol.16, No.5, 232-240 http://cropscipublisher.com/index.php/cgg 238 Figure 2 Reduced Gb-miR156/157 expression suppresses fiber elongation. (a) The mature fiber length of the samples planted in green house (2012) was measured manually with a comb. Control, transgenic plants containing the construct without the miRNA reverse complement site. Sensor156/157, transgenic plants of Gb-miR156/157 reverse complement site. (b) Relative expression of the Gb-miR156/157 target (TC253516) in fibers (12 DPA). R.E.L., the relative expression levels were calculated using GhUBQ7 as a control. (c) The mature fiber length of the samples planted in the experiment field (2013) was measured manually with a comb. Null, segregated nontransgenic plants derived from the three transformants MIM156/157-1, -2 and -3. MIM156/157, plants transformed with the miR156/157 mimicry vector. (d) The image of mature fiber. Error bars (a, c) represent standard deviation of samples from at least 20 ovules. Different letters in (a) and (c) indicate statistically significant differences at P < 0.05 based on analysis of variance (anova) (Tukey's Multiple Comparison Test). The error bars (b) indicate the standard deviation of three biological replicates (Adopted from Liu et al., 2014) 8 Concluding Remarks and Future Perspectives In cotton, is circRNA really the key regulatory factor? From the existing research, it is indeed involved in many important processes, especially in development and stress responses. It can adsorb miRNA, temporarily isolate these "small molecule regulators", and thereby affect the expression of target genes. This effect has been observed in multiple tissues and varieties. Especially under drought conditions, the "game relationship" among circRNA-miRNA-mRNA becomes more complex and even has a certain variety specificity. For instance, circ125 can bind to miR7484b and miR7450b and is believed to possibly play a certain regulatory role in stress adaptation. Although such discoveries are still rare, they have already begun to prompt us to rethink the "presence" of circrnas. Of course, many problems remain unsolved. At present, the role of circRNA-miRNA that has been truly verified through experiments is still very limited, and more is just prediction. As for whether these predictions are reliable or not, it still depends on whether conclusive evidence can be found in the future. The explanatory power of computational models is limited, and sometimes it is hard to tell whether it is a real biological interaction or an
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