Cotton Genomics and Genetics 2025, Vol.16, No.4, 192-201 http://cropscipublisher.com/index.php/cgg 195 Figure 1 Phenotypic analysis of fibres of GhMYB7 overexpression and RNA interference (RNAi)-silenced transgenic cotton plants. (a) Quantitative PCR analysis of GhMYB7 transcript levels in 18 d post anthesis (DPA) fibres of wild-type, GhMYB7 overexpression and RNAi-silenced transgenic lines from T3 generation. Cotton ubiquitin gene (GhUBI1) was used as an internal control for normalisation. Values represent mean ± SD of three biological replicates. OE12 and OE14, two independent lines from GhMYB7 overexpression plants; WT, wild-type; Ri9 and Ri28, two GhMYB7-RNAi-silenced transgenic cotton plants. Student’s t-tests demonstrated significant differences (*, P<0.05; **, P<0.01) between transcript levels of the transgenic lines and the wild-type. (b) Image of mature fibres from transgenic cotton lines and WT. Bar, 10 mm. (c) Statistical analysis of average mature fibre length of WT and transgenic lines. Values represent mean ± SD (n≥60). *, P<0.05; **, P<0.01. (d) Transmission electron microscopy analysis of the cell wall thickness of mature fibres of WT and transgenic lines. Bars, 10 µm. (e) Scanning electron microscopy images of the cellulose microfibrils arrangement on the surface of mature fibre in WT and transgenic lines. Bars, 10 µm. (f) Comparison of the median cell wall thickness of mature fibres from WT and transgenic lines shown in (d). Values represent mean ± SD (n≥10). *, P<0.05; **, P<0.01. (g) Crystalline cellulose content comparison of mature fibres between wild-type and transgenic lines. Values represent mean ± SD of three biological replicates. Student’s t-tests demonstrated that there were significant differences (*, P<0.05; **, P<0.01) between the transgenic lines and the wild-type. (h, i) Cross-sections of paraffin-embedded mature cotton fibres were stained with Pontamine Fast Scarlet 4B (S4B) and Calcofluor White to show cellulose distribution and abundance. Bars, 50 μm (Adopted from Huang et al., 2021)
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