Cotton Genomics and Genetics 2025, Vol.16, No.1, 12-20 http://cropscipublisher.com/index.php/cgg 17 quantities (Ahmed et al., 2024). In order for gene editing technology to be truly used in cotton breeding, it is necessary to improve transformation and regeneration methods. Only by solving these practical problems can the huge potential of gene editing in breeding stress-resistant varieties be truly realized. Figure 2 Schematic representation of genome editing mediated abiotic stress (drought, salinity, heat, cold, heavy metals) tolerance in plants. The model shows the stress-induced expression of the abiotic stress-responsive gene that leads to reduced plant biomass; photosynthetic rate; SOD, CAT, GPX, and PAL activities; and chlorophyll content and increased reactive oxygen species (ROS), flower and pod abortion, transpiration rate, ion leakage, and lipid peroxidation. Genome-edited knock-out/knock-in of stress-responsive genes resulted in broken/modified protein that modulates biochemical and physiological characteristics in plants and provides abiotic stress tolerance. SOD, superoxide dismutase; CAT, catalase; GPX, guaiacol peroxidase; PAL, phenylalanine ammonia-lyase; MDA, malondialdehyde; RWC, relative water content; EL, electrolytic leakage, As, Arsenic (Adopted from Kumar et al., 2023)
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