Molecular Soil Biology 2026, Vol.17, No.1, 1-11 http://bioscipublisher.com/index.php/msb 10 Acknowledgments This study was supported jointly by the Ministry of Education Innovation Team Science Fund Project (IRT_17R99) and the Heilongjiang Province College Student Innovation and Entrepreneurship Training Program Project (202410225509). Conflict of Interest Disclosure The authors declare no competing interests. References Abrha G.T., Makaranga A., and Jutur P.P., 2025, Enhanced lipid accumulation in microalgae Scenedesmus sp. under nitrogen limitation, Enzyme and Microbial Technology, 182: 110546. https://doi.org/10.1016/j.enzmictec.2024.110546 Balk J., and Schaedler A.T., 2014, Iron cofactor assembly in plants, Annual Review of Plant Biology, 65(1): 125-153. https://doi.org/10.1146/annurev-arplant-050213-035759 Brányiková I., Maršálková B., Doucha J., Brányik T., Bišová K., Zachleder V., and Vítová M., 2011, Microalgae-novel highly efficient starch producers, Biotechnology and Bioengineering, 108(4): 766-776. https://doi.org/10.1002/bit.23016 Chen Y., Zhu G., Sun Y.J., 2016, Biodiesel production from microalgal lipids catalyzed by Ca-Mg-Al composite metal oxides, Journal of Qingdao University of Science and Technology (Natural Science Edition), 37(3): 260-264. Cheng X., 2020, Response of photosynthesis in Chinese cabbage to rhizosphere phosphorus supply and regulation by calcium, Master's thesis, Shenyang Agricultural University, Advisor: Liu Y.F., pp.1-120. Chu Z.S., Jin X.C., Yan F., Zheng S.F., Pang Y., and Zeng Q.R., 2007, Effects of EDTA and iron on the growth and competition of Microcystis aeruginosa and Scenedesmus quadricauda, Environmental Science, (11): 2457-2461. Claudia O., and Jaime R., 2003, EDTA: the chelating agent under environmental scrutiny, Química Nova, 26(6): 901-907. https://doi.org/10.1590/S0100-40422003000600020 Fabris M., Abbriano R.M., Pernice M., Sutherland D.L., Commault A.S., Hall C.C., Labeeuw L., McCauley J., Kuzhiumparambil U., Chen H., Ralph P.J., 2020, Emerging technologies in algal biotechnology: toward the establishment of a sustainable, algae-based bioeconomy, Front. Plant Sci., 11(1): 279. https://doi.org/10.3389/fpls.2020.00279 Fan Y.C., Liu X.Y., Ni M.F., Wang Z.H., Zhang R.Y., Luo X.Y., Wang Z.K., 2025, Effect of calcium and magnesium on adsorption characteristics of extracellular polymeric substances for algal-derived organic matter, Chinese Journal of Environmental Science, 45(7): 3896-3904. Fawaz G.E., Salam A.D., Kamareddine L., 2018, Evaluation of copper toxicity using site specific algae and water chemistry: Field validation of laboratory bioassays, Ecotoxicology and Environmental Safety, 155: 59-65. https://doi.org/10.1016/j.ecoenv.2018.02.054 Gao L.L., 2024, Study on the effect of light and Ca2+/Mg2+ on algal-bacterial granular sludge, Master's thesis, Yangzhou University, Advisors: He Chengda, Huo Xiubing, pp.1-100. Geis S.W., Fleming K.L., Korthals E.T., Searle G., Reynolds L., and Karner D.A., 2000, Modifications to the algal growth inhibition test for use as a regulatory assay, Environmental Toxicology and Chemistry, 19(1): 36-41. https://doi.org/10.1002/etc.5620190105 Kona R., Hemalatha M., Srivastav K.V., and Venkata Mohan S., 2017, Regulatory effect of Fe-EDTA on mixotrophic cultivation of Chlorella sp. towards biomass growth and metabolite production, Bioresource Technology, 244(P2): 1227-1234. https://doi.org/10.1016/j.biortech.2017.06.028 Lei J.C., Li Z.J., Zhou J., Dai H.L., 2012, Application of active dry yeast in soy sauce, China Brewing, 31(6): 162-165. Markou G., and Nerantzis E., 2013, Microalgae for high-value compounds and biofuels production: A review with focus on cultivation under stress conditions, Biotechnology Advances, 31(8): 1532-1542. https://doi.org/10.1016/j.biotechadv.2013.07.011 Mishra S.K., Suh W.I., Farooq W., Moon M., Shrivastav A., Park M.S., and Yang J.W., 2014, Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method, Bioresource Technology, 155: 330-333. https://doi.org/10.1016/j.biortech.2013.12.077 Qiu W.S., 2021, Cultivation of Chlorella and co-production of lipids and paramylon, Master's thesis, Fujian Normal University, Advisor: Chen B.L., pp.1-110. Ren H.Y., Liu B.F., Kong F., Zhao L., Xie G.J., and Ren N.Q., 2014, Enhanced lipid accumulation of green microalga Scenedesmus sp. by metal ions and EDTA addition, Bioresource Technology, 169: 763-767. https://doi.org/10.1016/j.biortech.2014.06.062 Shin D.Y., Cho H.U., Utomo J.C., Choi Y.N., Xu X., and Park J.M., 2015, Biodiesel production from Scenedesmus bijuga grown in anaerobically digested food wastewater effluent, Bioresource Technology, 184: 215-221. https://doi.org/10.1016/j.biortech.2014.10.090 Thomas N.L., Dart C., and Helassa N., 2023, Editorial: The role of calcium and calcium binding proteins in cell physiology and disease, Frontiers in Physiology, 14: 1228885. https://doi.org/10.3389/fphys.2023.1228885
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