Molecular Soil Biology 2025, Vol.16, No.2, 73-82 http://bioscipublisher.com/index.php/msb 81 technologies can be used to introduce precise resistance traits, and marker assisted selection (MAS) can accelerate the breeding process by associating target traits with molecular markers (Huang, 2024). Genome selection (GS) combined with genome-wide markers can predict the breeding value of germplasm in complex resistance traits. By integrating these technologies, breeding programs can produce Germplasm with enhanced resistance, higher yield and better adaptation to Off-season Cultivation Conditions (Gu et al., 2010). Future work should also focus on the combination of molecular breeding and precision agriculture technology. Combining disease resistant germplasm with intelligent disease monitoring system and real-time environmental control can optimize cultivation practice and further improve the performance of disease resistant varieties (Li, 2024). By solving the technical bottlenecks and long-term challenges, these advances can pave the way for the sustainable large-scale cultivation of Leonurus japonicus in a diversified environment. Acknowledgments Thank you to the anonymous reviewers for their constructive and targeted suggestions for improving the manuscript. Conflict of Interest Disclosure The authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Behzadipour F., Ghasemi-Nejad-Raeini M., Mehdizadeh S., Taki M., Moghadam B., and Bavani M., 2024, Optimizing water use efficiency in greenhouse cucumber cultivation: A comparative study of intelligent irrigation systems, PLOS ONE, 19(10): e0311699. https://doi.org/10.1371/journal.pone.0311699 Buirs L., and Punja Z., 2024, Integrated management of pathogens and microbes in Cannabis sativa L. (Cannabis) under Greenhouse Conditions, Plants, 13(6): 786 https://doi.org/10.3390/plants13060786 Carolan K., Helps J., Van Den Berg F., Bain R., Paveley N., and Van Den Bosch F., 2017, Extending the durability of cultivar resistance by limiting epidemic growth rates, Proceedings of the Royal Society B: Biological Sciences, 284(1863): 20170828. https://doi.org/10.1098/rspb.2017.0828 Cho S., Park M., Shin C., and Shin H., 2013, First Confirmed Report of Powdery Mildew Caused by Podosphaera xanthii on Farfugium japonicum in Korea, Plant disease, 97(5): 691. https://doi.org/10.1094/PDIS-11-12-1069-PDN Cui B., Niu W., Du Y., and Zhang Q., 2020, Response of yield and nitrogen use efficiency to aerated irrigation and N application rate in greenhouse cucumber, Scientia Horticulturae, 265: 109220. https://doi.org/10.1016/j.scienta.2020.109220 Dahal K., Li X., Tai H., Creelman A., and Bizimungu B., 2019, Improving potato stress tolerance and tuber yield under a climate change scenario-a current overview, Frontiers in Plant Science, 10: 563. https://doi.org/10.3389/fpls.2019.00563 Gu Jingjing ., Ze-Xin J., and Junmin L., 2010, Genetic diversity of Lindera aggregata populations in Zhejiang province, Bulletin of Botanical Research, 30: 202-207. https://doi.org/10.7525/J.ISSN.1673-5102.2010.02.014 Gu J., Wu Y., Tian Z., and Xu H., 2020, Nitrogen use efficiency, crop water productivity and nitrous oxide emissions from Chinese greenhouse vegetables: A meta-analysis, The Science of the Total Environment, 743: 140696. https://doi.org/10.1016/j.scitotenv.2020.140696 Huang W.Z., 2024, The current situation and future of using GWAS strategies to accelerate the improvement of crop stress resistance traits, Molecular Plant Breeding, 15(2): 52-62. Jiang Y., Fu X., Wen M., Wang F., Tang Q., Tian Q., and Luo K., 2013, Overexpression of an nsLTPs-like antimicrobial protein gene (LJAMP2) from motherwort (Leonurus japonicus) enhances resistance to Sclerotinia sclerotiorum in oilseed rape (Brassica napus), Physiological and Molecular Plant Pathology, 82: 81-87. https://doi.org/10.1016/J.PMPP.2012.11.001 Jia Z., Gou J., Sun Y., Yuan L., Tang Q., Yang X., Pei Y., and Luo K., 2010, Enhanced resistance to fungal pathogens in transgenic Populus tomentosa Carr. by overexpression of an nsLTP-like antimicrobial protein gene from motherwort (Leonurus japonicus), Tree physiology, 30(12): 1599-605. https://doi.org/10.1093/treephys/tpq093 Koukounaras A., 2020, Advanced greenhouse horticulture: new technologies and cultivation practices, Horticulturae. 7(1): 1. https://doi.org/10.3390/horticulturae7010001
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