Molecular Soil Biology 2025, Vol.16, No.1, 45-54 http://bioscipublisher.com/index.php/msb 52 disease and other adverse environments. 8.3 Developing novel microbial combinations or biofertilizers to improve cultivation results In the future, efforts could be made to develop novel microbial communities or microbial-based fertilizers to improve the yield and quality of A. roxburghii. Current studies have shown that the use of soil amendments such as biochar can significantly impact the structure of rhizosphere microbes, improving soil conditions and promoting better A. roxburghii growth (Liu et al., 2022). In this regard, plant growth promoting bacteria (PGPR) are a group of bacteria that deserve attention. If they can be combined with fertilizers, they can enhance the efficiency of nutrient transformation and promote root development (Pii et al., 2015). Microorganisms that can dissolve phosphorus or produce iron carriers can also help Anoectochilus roxburghii absorb key nutrients and improve the overall state of the plant. If these plans can be implemented in practice, it will not only improve the cultivation efficiency, but also make the planting process more environmentally friendly and sustainable. 9 Conclusion Root microbes play an important role in helping Anoectochilus roxburghii grow, especially during tissue culture. Some helpful bacteria, like PGPR, can turn nutrients in the soil—such as phosphorus—into forms the plant can use. These bacteria also produce plant hormones like auxin, which help the roots grow better. As the roots develop, the whole plant gets stronger and makes more ginsenosides, which are the valuable medicinal compounds. Certain fungi, like Mortierella alpina, can also protect the plant by fighting off harmful pathogens like Fusarium, which causes root rot. In practice, using PGPR or beneficial fungi as inoculants is an effective way to improve the yield and quality of Anoectochilus roxburghii. These microorganisms can improve the nutrient supply in soil, enhance plant disease resistance, and improve the stability of soil microbial community. If combined with AMF biological fertilizer, it can further promote nutrient absorption and alleviate the negative impact of long-term repeated cultivation on soil. Reasonable application of NPK fertilizer can also indirectly promote the activity of beneficial bacteria and enhance the adaptability of plants to environmental stress. The connection between A. roxburghii and root microbes is about more than just helping the plant grow faster. These microbes can reduce the need for chemical fertilizers and pesticides, making farming more eco-friendly and efficient. But we still don’t fully understand how these microbes and plants work together. Future studies should look more closely at how these interactions happen at the genetic and ecological levels, especially under different growing conditions. This will help build better and more sustainable ways to growA. roxburghii. Acknowledgments Thank you to the project team for the careful guidance and strong support. Conflict of Interest Disclosure The author affirms that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Dong L., Xu J., Zhang L., Cheng R., Wei G., Su H., Yang J., Qian J., Xu R., and Chen S., 2018, Rhizospheric microbial communities are driven by Panax ginseng at different growth stages and biocontrol bacteria alleviates replanting mortality, Acta Pharmaceutica Sinica. B, 8: 272-282. https://doi.org/10.1016/j.apsb.2017.12.011 Dong Y., and Li Q.D., 2024, Screening and functional verification of pine rhizosphere nitrogen-fixing bacteria, Molecular Soil Biology, 15(2): 46-58. Fan S., Hu Q., Liu Q., Xu W., Wang Z., Huang Y., Zhang Y., Ji W., and Dong W., 2024, The synergistic effect of plant growth-promoting rhizobacteria and spent mushroom substrate improves ginseng quality and rhizosphere nutrients, Agriculture, 14(11): 1880. https://doi.org/10.3390/agriculture14111880 Fang X., Wang H., Zhao L., Wang M., and Sun M., 2021, Diversity and structure of the rhizosphere microbial communities of wild and cultivated ginseng, BMC Microbiology, 22(1): 2. https://doi.org/10.1186/s12866-021-02421-w
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