Molecular Soil Biology 2025, Vol.16, No.1, 45-54 http://bioscipublisher.com/index.php/msb 45 Research Insight Open Access The Study on the Promoting Effect of Rhizosphere Microorganisms on the Growth of Tissue-Cultured Ginseng and Its Mechanisms Chunyang Zhan Hainan Institute of Biotechnology, Haikou, 570206, Hainan, China Corresponding email: chunyang.zhan@hibio.org Molecular Soil Biology, 2025, Vol.16, No.1 doi: 10.5376/msb.2025.16.0005 Received: 10 Jan, 2025 Accepted: 12 Feb., 2025 Published: 27 Feb., 2025 Copyright © 2025 Zhan, This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Zhan C.Y., 2025, The study on the promoting effect of rhizosphere microorganisms on the growth of tissue-cultured ginseng and its mechanisms, Molecular Soil Biology, 16(1): 45-54 (doi: 10.5376/msb.2025.16.0005) Abstract This study looks at how microbes around the roots affect the growth of tissue-cultured Anoectochilus roxburghii (also known as golden thread orchid). Some helpful bacteria, called PGPR, and certain fungi can make it easier for plants to use nutrients in the soil. As a result, the plants grow bigger and produce more ginsenosides, which are valuable compounds. A fungus called Mortierella alpina also helps by stopping harmful germs from spreading, which lowers the chance of disease and keeps the plant healthy. The study also tried mixing PGPR, good fungi, and organic fertilizer. This mix helped the plants grow better and reduced the need for chemical fertilizers, which is better for the soil and environment. In the future, researchers can study more about how these microbes and plants work together. This could help improve how we grow golden thread orchid and support more eco-friendly farming. Keywords Rhizosphere microorganisms; Anoectochilus roxburghii tissue culture; Growth promotion; ginsenosides; Microbial inoculation 1 Introduction Anoectochilus roxburghii is a kind of Chinese herbal medicine, which is widely used in traditional Chinese medicine, especially the ginseng type in tissue culture seedlings. It contains a variety of effective ingredients, such as ginsenosides, polysaccharides and peptides, which can resist oxidation, reduce inflammation and enhance immunity, so it is often used to treat diseases such as poor physical strength, loss of appetite and diabetes, and has been used in China for thousands of years (Xiang et al., 2008). As both modern and traditional medicine continue to demand more Anoectochilus roxburghii, researchers have turned their attention to tissue culture as a way to boost production. This method allows plants to be grown under controlled lab conditions to produce key compounds such as saponins and polysaccharides (Wu and Zhong, 1999; Xu et al., 2023). Common approaches include callus culture, somatic embryogenesis, and cell suspension culture. Compared to traditional farming, these techniques are more efficient and easier to manage (Qiang et al., 2020; Xu et al., 2023). Conventional methods are time-consuming, labor-intensive, and often unstable (Wu and Zhong, 1999). Extended periods of tissue culture may cause problems, such as chromosome instability or loss of cell differentiation ability, which can affect results (Liu et al., 2021). How to adjust the culture environment, formula and conditions so that plant cells can produce more needed metabolites is still the focus of attention (Jing, 2024; nhung et al., 2024). This study explores a new approach: using rhizosphere microbes to promote the growth of tissue-cultured A. roxburghii. It focuses on how these microbes interact with the plant and how they can be used to improve the culture system. The goal is to raise both yield and active compound levels while addressing current problems in tissue culture, paving the way for more stable and sustainable production in the future.
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