Molecular Soil Biology 2025, Vol.16, No.1, 37-44 http://bioscipublisher.com/index.php/msb 41 cultivation method worth trying and is suitable for production needs that require stable yield and quality (Zhang et al., 2023). 6 Case Studies and Experimental Findings onLeonurus japonicus 6.1 Domestic and international research on the impact of environmental factors onLeonurus japonicus Environmental conditions have a great influence on the growth and medicinal components of Leonurus japonicus. In China, the pH of soil can regulate the synthesis of alkaloids. In neutral or slightly alkaline soils, the nitrogen metabolism of plants is smoother, and the content of active ingredients such as carnosine is also higher (Zhang et al., 2022). Cha (2021) pointed out that the activity of some enzymes in plants will increase under stress, which can improve the synthesis ability of antioxidants and the stress tolerance of plants (Figure 2). Figure 2 (A) HPLC chromatogram of rutin and quercetin, (B) HPLC chromatography of MeOH extract of Leonurus japonicus Houttuyn, (C) HPLC chromatogram of viscozyme (1%, 24 hr) reaction product of Leonurus japonicus Houttuyn (Adopted from Cha, 2021) International research has focused on the role of light and temperature. Miao et al. (2019) in Japan found that increasing light intensity during the critical growth period significantly increased the content of flavonoids and phenolic acids, enhancing the antioxidant properties of the plant. Kuchta et al. (2013) in Europe noted that climate instability can affect the synthesis of secondary metabolites in Leonurus japonicus, and maintaining stable environmental conditions can stabilize yield. 6.2 Findings from laboratory and field trials Tan et al. (2018) conducted hydroponic experiments showing that varying nutrient concentrations affect the levels of carnosine and leonurine. When water stress is mild, flavonoid production increases, presumably as a stress response (Shen et al., 2002). Xu et al. (2006) conducted research in China showing that well-drained, nutrient-rich soil and appropriate light conditions can increase the yield and active ingredient content of Leonurus viridis. Furthermore, Shang et al. (2014) conducted comparative field trials in Vietnam, finding that under moderate humidity and stable
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