Molecular Soil Biology 2024, Vol.15, No.1, 17-27 http://bioscipublisher.com/index.php/msb 19 community size at various stages from inoculation to the rhizosphere, the researchers assessed the effectiveness of SynComs. The study also constructed a phylogenetic tree of the 36 selected strains and illustrated the composition of the 13 SynComs, indicating their richness and the relative abundance of each strain in the original seed samples. Notably, seven strains were included in the study despite being undetected by the metabarcoding approach. This highlights the necessity of employing diverse methods, combining traditional microbiology with advanced genetic techniques, to gain a comprehensive understanding of microbial community dynamics and their application in sustainable agriculture. Figure 1 Design of the different experiments, strain selection, and SynCom compositions (Adopted from Arnault et al., 2024) Image caption: A) Overview of the different experiments. B) Phylogenetic tree of the 36 strains selected and composition of the 13 SynComs. The number in SynCom names indicates the SynCom richness. Relative abundance and prevalence of each strain in the original seed samples are plotted on the right side. Seven strains were selected while they were not detected using the metabarcoding approach (Adopted from Arnault et al., 2024) 2.2 Techniques for engineering SynComs The engineering of SynComs involves several advanced techniques, including:Synthetic Biology, This approach involves the design and construction of new biological parts, devices, and systems. Synthetic biology can be used to create novel metabolic pathways in microorganisms, enabling them to degrade pollutants or produce beneficial compounds. Genetic Modification: Genetic engineering techniques, such as CRISPR-Cas9, are employed to introduce specific genes into microbial genomes. These genes can confer traits such as salt tolerance, heavy metal resistance, or enhanced nutrient uptake. Metagenomics and Metatranscriptomics techniques allow for the
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