Journal of Energy Bioscience 2024, Vol.15, No.2, 72-84 http://bioscipublisher.com/index.php/jeb 80 Figure 2 Effect of Enzyme (Lipozyme TL IM) Concentration on the Degree of Transesterification and Triglyceride Content in Biodiesel (Adapted from Santaraite et al., 2020) Image Description: Experimental conditions: oil to ethanol molar ratio of 1:5, reaction time of 7 hours, reaction temperature of 30°C (Adapted from Santaraite et al., 2020) 7.2 Genetic improvements and agricultural practices for higher yield and oil content Genetic engineering and advanced breeding techniques have been pivotal in developing rapeseed varieties with higher oil content and better agronomic traits. For instance, transgenic rapeseed varieties have been engineered to express novel seed oils and proteins, leading to improved seed-oil yield and quality. Marker-assisted selection has also been employed to develop high oleic and low linolenic acid rapeseed lines, which are ideal for both biodiesel production and agricultural performance (Spasibionek et al., 2020). Furthermore, optimized fertilization systems have been shown to significantly increase rapeseed yield and oil content, thereby enhancing the overall efficiency of biodiesel production (Figure 3) (Lovasz et al., 2023). Figure 3 Regression relationship between different fertilization combinations and rapeseed yield (q ha-1) (Adapted from Lovasz et al., 2023) Lovasz et al. (2023) studied the regression relationship between different fertilization combinations and rapeseed yield (q ha-1). The fertilization amount increased significantly from no (NoPoKo) to gradually increasing (N160P160K160 to N480P480K480), resulting in a significant increase in rapeseed yield. This proves that fertilization has a positive impact on the growth of rapeseed, not only increasing yield, but also potentially increasing the oil content of rapeseed, providing effective yield increasing measures for improving the overall efficiency of biodiesel production.
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