Genomics and Applied Biology 2026, Vol.17, No.1, 1-15 http://bioscipublisher.com/index.php/gab 3 uses biotin-labeled specific primers to amplify target genomic regions. The amplified fragments are then captured via biotin binding, eluted, and sequenced. Notable examples of this approach include AmpliSeq and GenoPlexs. The GBTS technology enables the capture of multiple SNPs (mSNPs) within a single amplicon. This mSNPs strategy allows simultaneous acquisition of multiple SNPs information in a single sequencing run, thereby yielding richer data that facilitates haplotype analysis. Xu et al. (2020) reviewed the principles and applications of two GBTS technologies-GenoBaits (Figure 1) and GenoPlexs-outlined (Figure 2) the workflow of the mSNPs strategy, provided a comparative analysis with genotyping by sequencing technology, and offered valuable references for the broader adoption of GBTS. It is noteworthy that SNP data acquisition is a prerequisite for gene chip development. In the development of the rice C6AIR array (Thomson et al., 2017) and soybean NJAU 355K SoySNP array (Wang et al., 2016), selected varieties were resequenced to obtain SNP information. The Rice3K56 SNP array (Zhang et al., 2023) and Wheat Breeders’ Array (Allen et al., 2017) screened candidate SNPs based on previous studies according to research objectives. The rice 580K_KNU chip (Kim et al., 2022) integrated existing SNP databases and filtered key SNP loci for development. Figure 1 Flowchart for genotyping by target sequencing with GenoBaits (Adopted from Xu et al., 2020)
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