Genomics and Applied Biology 2024, Vol.15, No.4, 200-211 http://bioscipublisher.com/index.php/gab 207 Figure 3 Phenotypes of the OsMYB60-overexpressing (OsMYB60-OE) transgenic plants (Adopted from Liu et al., 2018) Image caption: (a) Phenotypes of 100-d-old wild-type (WT, left) and OsMYB60-OE (right) transgenic rice plants. (b) Flag leaf blades of 100-d-old WT (left) and OsMYB60-OE (right: #1, #3 and #6) transgenic rice plants. OsMYB60-OE transgenic rice plants showed twisted leaf blades. (c) Histological analysis of the leaf blade in the middle part of WT and OsMYB60-OE transgenic rice plants. The shape and arrangement of bulliform cells in the OsMYB60-OE leaves were changed compared to WT. (d) OsMYB60 expression in OsMYB60-OE transgenic rice plants was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The Rice ACTIN1 gene was used as an internal control. Error bars show ± standard deviation from three replicates. Asterisks indicate a significant difference between OsMYB60-OE transgenic plants and WT controls by Student's t-test: ***, P < 0.001. Bars: (a) 20 cm; (b) 5 cm; (c) 25 μm (Adopted from Liu et al., 2018) 8.2 Potential for genetic engineering and crop improvement The identification and characterization of R2R3-MYB genes have opened new avenues for genetic engineering aimed at crop improvement. For example, the overexpression of R2R3-MYB genes, such as OsMYB2P-1, has been demonstrated to enhance tolerance to phosphate starvation and improve root architecture in rice (Dai et al., 2012). Similarly, OsMYB103L has been demonstrated to influence leaf rolling and mechanical strength, which are critical traits for rice breeding (Yang et al., 2014). Genetic engineering techniques can be utilized to alter these genes, thereby developing rice varieties with enhanced stress tolerance, improved nutrient use efficiency, and desirable morphological characteristics. Furthermore, the distinctive allelic variations observed in purple rice landraces can be utilized to incorporate advantageous traits, such as enhanced nutritional value and stress resistance, into contemporary cultivars through targeted breeding programs (Wang and Shu, 2007; Lachagari et al., 2019).
RkJQdWJsaXNoZXIy MjQ4ODYzMg==