Genomics and Applied Biology 2024, Vol.15, No.4, 182-190 http://bioscipublisher.com/index.php/gab 186 Figure 3 The phenotypes of ospin5b/gs3/osmyb30-4, ospin5b/gs3/osmyb30-25 and the predicted protein structure of OsPIN5b and OsMYB30 inospin5b/gs3/osmyb30-4, ospin5b/gs3/osmyb30-25(Adopted from Zeng et al., 2020) Image caption: (A) Whole plant morphology, survival rate after cold treatment and grain size in WT, ospin5b/gs3/osmyb30-4, ospin5b/gs3/osmyb30-25. Bar = 20 cm. (B) The OsPIN5b and OsMYB30 protein structures of WT, ospin5b/gs3/osmyb30-4 and ospin5b/gs3/osmyb30-25 were predicted by SWISS-MODEL. The observed differential regions were highlighted in green (Adopted from Zeng et al., 2020) 5 Challenges and Limitations of CRISPR/Cas9 in Rice Yield Improvement 5.1 Off-target effects and genetic stability One of the primary challenges associated with the use of CRISPR/Cas9 in rice yield improvement is the potential for off-target effects. Although CRISPR/Cas9 is known for its precision, unintended mutations can occur at sites with similar sequences to the target, leading to genetic instability. Studies have shown that while the
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