Genomics and Applied Biology 2024, Vol.15, No.4, 182-190 http://bioscipublisher.com/index.php/gab 185 Figure 2 Scheme of short breeding cycle using CRISPR/Cas9 in rice. Agrobacterium-mediated transformation used for genome editing with CRISPR/Cas9. In regenerated plants, a gene was selected and edited by T-DNA insertion and sequencing. On the T0 plant, each spike is assigned a different accession number. T1 plants are transplanted to one row for each line. T2 seeds are harvested in bulk (Adopted from Park et al., 2022) 4.3 Reducing susceptibility to environmental stress CRISPR/Cas9 has also been instrumental in developing rice varieties with reduced susceptibility to environmental stress. For instance, editing the OsMYB30 gene, which is associated with cold tolerance, has resulted in rice mutants with enhanced resilience to cold stress. These mutants, when combined with edits in other yield-related genes like OsPIN5b and GS3, not only showed improved stress resistance but also maintained high yield traits. This dual improvement in yield and stress tolerance demonstrates the versatility and effectiveness of CRISPR/Cas9 in addressing multiple breeding goals simultaneously (Zeng et al., 2020). Furthermore, the technology has been applied to develop rice varieties that can sustain high yields under various biotic and abiotic stresses, ensuring food security in the face of climate change and other environmental challenges (Abdelrahman et al., 2018; Haque et al., 2018).
RkJQdWJsaXNoZXIy MjQ4ODYzMg==