Genomics and Applied Biology 2024, Vol.15, No.1, 22-26 http://bioscipublisher.com/index.php/gab 25 3.3 Prospects As a fourth-generation sequencing technology, nanopore sequencing offers advantages such as low cost, long reads, and ease of integration onto chips. With the rapid development of semiconductor technology, it is now possible to manufacture high-speed, high-throughput nanopore gene sequencing microchips (Chen et al., 2014). Furthermore, the latest technological innovations of Oxford Nanopore Technologies (ONT) hold promise for addressing the implementation challenges in clinical laboratories, such as high costs, lengthy result times, and the demand for specialized technical and bioinformatics expertise. The ONT sequencing platform has become an attractive option for clinical laboratories due to its low cost, fast turnaround time, and user-friendly bioinformatics workflow (Petersen et al., 2019). In summary, despite facing a series of challenges, portable nanopore sequencing technology still has a very broad prospect in genomic research and clinical diagnostics. As the technology continues to advance and optimize, these challenges will be gradually overcome, and nanopore sequencing is expected to become an important tool for future genetic sequencing. References Bayley H., 2015, Nanopore sequencing: from imagination to reality, Clinical chemistry, 61(1): 25-31. https://doi.org/10.1373/clinchem.2014.223016 Benítez-Páez A., and Sanz Y., 2017, Multi-locus and long amplicon sequencing approach to study microbial diversity at species level using the MinION™ portable nanopore sequencer, GigaScience, 6: 1-12. https://doi.org/10.1101/117143. Benítez-Páez A., Portune K., and Sanz Y., 2015, Species-level resolution of 16S rRNA gene amplicons sequenced through the MinION™ portable nanopore sequencer, GigaScience, 5. https://doi.org/10.1186/s13742-016-0111-z Chen P., Sun Z., Wang J., Liu X., Bai Y., Chen J., Liu A., Qiao F., Chen Y., Yuan C., Sha J., Zhang J., Xu L., and Li, J., 2023, Portable nanopore-sequencing technology: Trends in development and applications, Frontiers in Microbiology, 14. https://doi.org/10.3389/fmicb.2023.1043967 Chen W., Luo J., and Zhao C., 2014, Solid-State Nanopore:The Next-Generation Sequencing Technology——Principles, Fabrication and Challenges, 44: 649-662 https://doi.org/10.1360/N052014-00091 Cummings P., Olszewicz J., and Obom K., 2017, Nanopore DNA Sequencing for Metagenomic Soil Analysis, Journal of Visualized Experiments: JoVE https://doi.org/10.3791/55979 Hall C., Zascavage R., Sedlazeck F., and Planz J., 2020, Potential applications of nanopore sequencing for forensic analysis, Forensic Science Review, 32(1): 23-54. Liu Z., Wang Y., Deng T., and Chen Q., 2016, Solid-State Nanopore-Based DNA Sequencing Technology, Journal of Nanomaterials, 5284786. https://doi.org/10.1155/2016/5284786 Magi A., Semeraro R., Mingrino A., Giusti B., and D’Aurizio R., 2017, Nanopore sequencing data analysis: state of the art, applications and challenges, Briefings in Bioinformatics, 19(6): 1256-1272. https://doi.org/10.1093/bib/bbx062 McIntyre A., Rizzardi L., Yu A., Rosen G., Alexander N., Botkin D., John K., Castro-Wallace S., Burton A., Feinberg A., and Mason C., 2015, Nanopore Sequencing in Microgravity, bioRxiv. https://doi.org/10.1101/032342 Pareek C., Smoczyński R., and Tretyn A., 2011, Sequencing technologies and genome sequencing, Journal of Applied Genetics, 52: 413-435. https://doi.org/10.1007/s13353-011-0057-x Petersen L., Martin I., Moschetti W., Kershaw C., and Tsongalis G., 2019, Third-generation sequencing in the clinical laboratory: exploring the advantages and challenges of nanopore sequencing, Journal of Clinical Microbiology, 58. https://doi.org/10.1128/JCM.01315-19 Pomerantz A., Peñafiel N., Arteaga A., Bustamante L., Pichardo F., Coloma L., Barrio-Amorós C., Salazar-Valenzuela D., and Prost S., 2018, Real-time DNA barcoding in a rainforest using nanopore sequencing: opportunities for rapid biodiversity assessments and local capacity building, GigaScience, 7. https://doi.org/10.1093/gigascience/giy033 Rhee M., and Burns M., 2006, Nanopore sequencing technology: research trends and applications, Trends in biotechnology, 24(12): 580-586. https://doi.org/10.1016/J.TIBTECH.2006.10.005 Samarakoon H., Punchihewa S., Senanayake A., Hammond J., Stevanovski I., Ferguson J., Ragel R., Gamaarachchi H., and Deveson I., 2020, Genopo: a nanopore sequencing analysis toolkit for portable Android devices, Communications Biology, 3. https://doi.org/10.1038/s42003-020-01270-z
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