Genomics and Applied Biology 2024, Vol.15, No.1, 22-26 http://bioscipublisher.com/index.php/gab 22 Technical Review Open Access Portable Nanopore Sequencing Technology: A Revolutionary Progress in Bioinformatics JimManson The HITAR institute Canada, Vancouver, BC, Canada Corresponding email: Jim.mason@hitar.org Genomics and Applied Biology, 2024, Vol.15, No.1 doi: 10.5376/gab.2024.15.0004 Received: 23 Nov., 2023 Accepted: 28 Dec., 2023 Published: 10 Jan., 2024 Copyright © 2024 Manson, This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Manson J., 2024, Portable nanopore sequencing technology: a revolutionary progress in bioinformatics, Genomics and Applied Biology, 15(1): 22-26 (doi: 10.5376/gab.2024.15.0004) Abstract With the rapid development of genomic science, portable nanopore sequencing technology has become a revolutionary progress in the field of bioinformatics due to its unique portability, real-time capabilities, and high-throughput sequencing capacity. This paper comprehensively analyzes the principles and characteristics of portable nanopore sequencing technology and its wide applications in environmental monitoring, epidemic prevention and control, and on-site rapid diagnosis. Through practical cases, it demonstrates how this technology provides efficient and real-time new solutions for biological research and public health emergency response. Meanwhile, it discusses the challenges faced by this technology, including data accuracy, sequencing costs, and data processing issues, and looks forward to the future development trends and application prospects. Portable nanopore sequencing technology not only promotes research in the field of bioinformatics but also has a profound impact on the formulation of public health monitoring and disease control strategies. Keywords Portable nanopore sequencing; Bioinformatics; Environmental monitoring; Epidemic prevention and control; Rapid diagnosis; Public health emergency response Introduction Since the development of the first generation of DNA sequencing technology by Frederick Sanger in 1977, genome sequencing technologies have evolved rapidly. From the initial Sanger sequencing to the second generation of high-throughput sequencing (HTS) technologies (Pareek et al., 2011), and to the recently developed third generation sequencing technologies such as single-molecule real-time sequencing (SMRT) and nanopore sequencing technology (Yanhu et al., 2015), there have been qualitative leaps in the speed, cost, and accuracy of genome sequencing. The advancements in genome sequencing technologies have not only accelerated research in life sciences, facilitated the implementation of precision medicine and personalized treatments, but also shown tremendous potential applications in fields such as agricultural improvement, microbiology, and environmental science (Zheng et al., 2023). With the advancement of technology and increasing research demands, higher requirements have been placed on genome sequencing technologies, particularly in terms of flexibility, speed, and portability (Chen et al., 2023). The advent of portable nanopore sequencing technology, which allows for direct sequencing of DNA and RNA using miniaturized devices, enables its use outside of the laboratory and provides real-time sequencing data (Samarakoon et al., 2020). The development of this technology marks a new era in genomics research, making it possible to perform genome sequencing in field environments, clinical settings, and even in space (Pomerantz et al., 2018). Portable nanopore sequencing technology has attracted widespread attention in the field of bioinformatics due to its unique technical advantages. Its innovation lies not only in the portability of the equipment but also in its ability to provide long reads, which are crucial for solving complex genomic regions assembly, identifying repetitive sequences, and detecting epigenetic modifications. Additionally, the capability of this technology to analyze data in real-time offers new solutions for rapid response to public health events, environmental monitoring, and on-site diagnostics, showcasing its broad application prospects across multiple fields.
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