Computational Molecular Biology 2025, Vol.15, No.5, 245-253 http://bioscipublisher.com/index.php/cmb 24 8 Figure 1 RagTag enables rapid generation of new reference genomes for the tomato genotypes Sweet-100 and M82 (Adopted from Alonge et al., 2022) 5 Case Study in Place: CRISPR Editing in Tomato 5.1 Objective: functional disruption of SlMLO1 gene using algorithm-assisted CRISPR design In the research on powdery mildew in tomatoes, the SlMLO1 gene has always been regarded as a typical "susceptibility gene". As long as it is deprived of function, tomatoes can acquire natural resistance to the powdery mildew pathogen Oidium neolycopersici. It is not intended to introduce exogenous DNA here. Instead, it is hoped to directly create damage similar to natural mutations on this gene through CRISPR/Cas9, such as the 19 bp
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