Computational Molecular Biology 2025, Vol.15, No.1, 38-52 http://bioscipublisher.com/index.php/cmb 47 strike. In this genetic "chaos", transcription factors such as MYB and NAC are like command centers, busy coordinating the emergency response of downstream genes. However, there are also "hindrance" ones, such as BnA.JAZ5 (Figure 4) (Cao et al., 2022). By interfering with ABA and JA hormone signals, it not only causes dysregulation of stomatal density but also reduces the seed germination rate. These findings indicate that the drought resistance of rapeseed cannot be resolved by just one or two genes, but is the result of the combined effect of the entire gene network-some genes charge ahead while others secretly create obstacles. This complex interaction ultimately determines the drought tolerance of the plant. Figure 4 Drought resistance testing of p35S::BnA.JAZ5 plants at the five-leaf stage. (A) Drought resistance assay of five overexpressing lines. (B) Survival rates of five overexpressing lines after 3-day recovery from drought stress treatment. WT, accession K407. OE-1, OE-2, OE-3, OE-4 and OE-5, p35S::BnA.JAZ5 plants. Asterisks indicate significant differences between wild-type and p35S::BnA.JAZ5 lines (p < 0.05, Student’s t-test). Scale bar, 10 cm (Adopted from Cao et al., 2022) 8.3 Potential breeding and genetic engineering solutions To cultivate drought-resistant rapeseed varieties, researchers have found several breakthroughs. That disruptive BnA.JAZ5 gene has instead become an important clue-regulating its expression may improve the stomatal response of rapeseed. More interestingly, lncrnas that are abnormally active during drought; In 2020, they act as
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