Computational Molecular Biology 2025, Vol.15, No.1, 26-37 http://bioscipublisher.com/index.php/cmb 31 5.2 Sprouting regulation networks 5.2.1 Hormonal signals affecting sprouting StABI3 and ABA work together seamlessly to manage the sleeping of tubers. As soon as ABA increases, StABI3 immediately enters a working state, like a strict conduit, tightly closing the gate of cell growth (Wang et al., 2020). But don't be fooled by its current dominance, when the tubers should wake up, it will slip faster than anyone else. The cooperation between these two is simply amazing-ABA is like a duty manager, calling StABI3, the 'night shift security guard', to work at the right time; Wait for the sleep period to end and let the security guard off work on time. The most wonderful thing is that this mechanism is particularly intelligent, neither making the tuber oversleep nor waking it up early, more reliable than any smart alarm clock. If it weren't for their precise regulation, the tubers would either not wake up or wake up too early, which would make storage very troublesome. 5.2.2 Role of StPIN proteins in hormone transport StPIN4, the 'transport team leader', is really a key role, as it is specifically responsible for moving auxin around the tubers. Interestingly, its performance also depends on the expression of protein kinases such as StCDPK1only when they are phosphorylated and activated can they function properly (Santin et al., 2017). This is like a sophisticated logistics system: where and how much growth hormone should be sent depends on StPIN4 scheduling. If this transportation system goes wrong, the trouble will be big-the tubers will either be stuck in bed or grow crooked. Most importantly, this transportation network is also subject to various post transcriptional regulation to ensure that auxin can appear in the correct position at the critical moment of dormancy and germination. So, if you want the tubers to sleep well and wake up cleverly, StPIN4, the 'delivery guy', must not fall off guard. 5.3 Research case: effect of temperature on potato dormancy duration 5.3.1 The impact of environmental variables on dormancy maintenance In a low-temperature environment (2-4℃), the 'Favorita' potato tubers seem to have been hit by the pause button-the ABA content increases sharply, freezing the cell activities (Di et al., 2019). This is particularly interesting at this time. All the growth-promoting hormones have collectively gone on hold: the DWF1 and BRI1 genes of the BR hormone have wilted, and the synthesis of GA has also been blocked, just like a hibernating little animal. But when the temperature rises above 25℃, the situation is completely reversed: the ABA content drops sharply, and the "alarm hormones" like GA and ethylene start to output like crazy. Even StABI3, the hibernation manager, leaves work early. However, the sprouts produced by high temperatures often lack stamina because the nutrient reserves in the tubers are consumed too quickly, and the new plants that eventually grow are always wilted and droopy. This is just like forcibly pulling a sleeping person up to work. How could the efficiency be high? 5.3.2 Experimental results for the 'favorita' variety It's quite interesting to conduct experiments with the variety 'Favorita'-the tubers were stored at different temperatures of 2℃, 12℃and 25℃respectively, and the results varied greatly. The coldest group (2℃) was just like being frozen. It didn't sprout for over 120 days. The test found that the ABA hormone was outrageously high, while the GA hormone was pitifully low. However, when the temperature is adjusted to 12℃, it's completely different. The dormancy period is directly cut by one third, and buds start to sprout around 90 days later, with the GA level also rising accordingly. The most exaggerated one was the group at 25℃, where the GA hormone seemed to have been injected with adrenaline. Unfortunately, I didn't remember all the data. These results do confirm the influence of temperature on the sleeping duration of tubers. However, strangely enough, in the same batch of experiments, there were always a few tubers that did not follow the usual pattern, not waking up when they should and just joining in the fun when they shouldn't. 5.3.3 Research case: the influence of temperature on the duration of potato dormancy As soon as the temperature reaches 25 ℃, the 'Favorita' tubers can no longer hold on and quickly sprout after about 30 days (Di et al., 2019). At this moment, ABA seemed to have let out its breath and fell down, while GA
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