Bioscience Methods 2025, Vol.16, No.6, 270-279 http://bioscipublisher.com/index.php/bm 2 72 Some people began to try slow-release and controlled-release fertilizers (SCRF) - such as those coated fertilizers or granular mixed types - the advantage is that the fertilization rhythm is more in line with the needs of rice, the dosage is more precise, and the effect is much more stable. Many experiments and data summaries show that such practices can increase the yield by 6% to 24%, and the efficiency of nitrogen fertilizer utilization also improves accordingly, with a maximum increase of 53% (Jiang et al., 2023). Of course, to be fair, although these new types of fertilizers are good, their costs and technical thresholds are indeed not low, and they are not so easy to promote. Figure 1 Identification and functional validation of the transcription factor OsNAC42 on chromosome 9. a Local Manhattan plot (top) and LD heatmap (bottom) surrounding the peak on chromosome 9. Red dashed lines indicate the candidate region for the peak. b Comparative analyses of OsNAC42 between the low and high NUE haplotypes. Boxplots for NPHR (normalized plant height ratio)-2014 (top) and NPHR-2016 (bottom) based on the haplotypes (Hap) for OsNAC42. Box edges represent the 0.25 quantile and 0.75 quantile with the median values shown by bold lines. Whiskers extend to data no more than 1.5 times the interquartile range, and remaining data are indicated by dots. Differences between the haplotypes were analyzed by Welch’s t test. c Phenotype of WT (Zhonghua11, ZH11), Tilling mutant (osnac42) and heterozygote (H), bar = 20 cm. d qRT-PCR analysis of OsNPF6.1 of WT (Zhonghua11, ZH11), Tilling mutant (osnac42) and heterozygote (H). Three biological replicates were used for qRT-PCR. e OsNPF6.1 and OsNAC42 transcript levels in different tissues. OsActin1 was used as a control, n = 3. f OsNPF6.1 and OsNAC42 expression under HN treatment from 0 to 24 h in leaf (KCl as a control of LN representing nitrogen starvation), n = 3. Data are presented as means ± SD. P values (versus the ZH11) were calculated with Student’s t test. **P < 0.01. The source data underlying (Adopted from Tang et al., 2019) 3.2 Organic-integration fertilization and the promotion of ecological farming When it comes to sustainable farming, relying solely on chemical fertilizers is probably not a long way off. Many places have begun to try to bring back the "traditional old methods" such as manure, compost and bio-organic fertilizer, and use them together with inorganic fertilizer. The results are really good. Not only can the yield be
RkJQdWJsaXNoZXIy MjQ4ODYzNA==