BM_2025v16n6

Bioscience Methods 2025, Vol.16, No.6, 270-279 http://bioscipublisher.com/index.php/bm 2 70 Review Article Open Access Field Evaluation of Nitrogen-use Efficient Rice Varieties under Varying Fertilizer Regimes Deshan Huang, Ruchun Chen, Dapeng Zhang Hier Rice Research Center, Hainan Institute of Tropical Agricultural Resources, Sanya, 572025, Hainan, China Corresponding email: dapeng.zhang@hitar.org Bioscience Methods, 2025, Vol.16, No.6 doi: 10.5376/bm.2025.16.0026 Received: 03 Sep., 2025 Accepted: 12 Oct., 2025 Published: 02 Nov., 2025 Copyright © 2025 Huang et.al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Huang D.S., Chen R.C., and Zhang D.P., 2025, Field evaluation of nitrogen-use efficient rice varieties under varying fertilizer regimes, Bioscience Methods, 16(6): 270-279 (doi: 10.5376/bm.2025.16.0026) Abstract Improving the nitrogen use efficiency (NUE) of rice is of great significance for ensuring crop yield while reducing the environmental impact caused by excessive nitrogen application. This study focuses on the field performance assessment of high-nitrogen-efficient rice varieties under different fertilization patterns, covering strategies such as conventional fertilization, reduced fertilization, slow-release fertilization, and organic combined fertilization. It analyzes the performance of the varieties in terms of nitrogen absorption, utilization efficiency, and yield composition, and screens the varieties suitable for the development of green agriculture - the optimal fertilization combination Through field trials conducted in major rice-growing areas in China (such as the middle and lower reaches of the Yangtze River, the main production areas in Northeast China, and the double-cropping rice-growing areas in South China), the responses and adaptability of different varieties to fertilization patterns were compared. Research has found that different rice varieties with high nitrogen efficiency show significant differences under different nitrogen application conditions, indicating that species-specific fertilization optimization is of great significance for improving nitrogen recovery rate and yield. This study provides a theoretical basis and practical path for constructing an efficient and low-input rice cultivation system, and offers scientific support for subsequent research on the genotype-fertilization interaction mechanism and the formulation of green agricultural policies. Keywords Rice; Fertilization mode; Nitrogen use efficiency (NUE); Field experiment; Green agriculture 1 Introduction For many people, a bowl of rice is nothing, but on a global scale, rice is a staple food that more than half of the population cannot do without every day. It is precisely for this reason that in order to maintain a stable job in the future, production must be increased. Among them, nitrogen (N) is an unavoidable keyword - it is the main "booster" for the growth and development of rice, and has a direct impact on both yield and quality. For decades, by continuously increasing nitrogen application, the total output of rice has indeed been pushed up. However, problems have also emerged: only about 40% to 50% of the fertilizer applied can be absorbed and utilized by rice, while the rest either seeps into water, drifts into the air, or becomes greenhouse gases, ultimately affecting farmers' income. While ensuring stable production and supply, it is also necessary to safeguard the environmental bottom line. At this point, "improving nitrogen fertilizer utilization rate" has truly become a hard indicator in the global rice-growing system. In recent years, researchers have not been idle either. It seems a feasible approach to make changes in breeding by increasing the nitrogen fertilizer utilization rate (NUE) to relieve the pressure. Relevant genes, such as those responsible for nitrogen absorption, transport and assimilation, are being identified one by one and attempts are being made to introduce them into existing superior varieties (Hu et al., 2022; Wang et al., 2022; Xin et al., 2025). With the increasing maturity of tools such as molecular breeding and GWAS, the breeding efficiency is also accelerating, which is expected to bring those new varieties that are "fertilizer-saving and high-yielding" to the fields earlier. But relying solely on the variety is not enough; it also depends on whether the fertilization plan can keep up. Only with the "dual-wheel drive" of variety and management can the biological potential be truly transformed into stable production and environmental protection.

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