BM_2025v16n4

Bioscience Methods 2025, Vol.16, No.4, 204-217 http://bioscipublisher.com/index.php/bm 207 rice OsGS1;1 has led to an imbalance in carbohydrate distribution and a decrease in yield (Figure 1) (Liu et al., 2022). Therefore, moderate expression and spatiotemporal regulation of key enzyme genes are conducive to improving overall nitrogen efficiency. There are many genes that determine the efficiency of nitrogen absorption, assimilation and recycling in rice, including transporters, metabolic enzymes, etc. Figure 1 Identification and functional validation of OsGS1;1 on chromosome 2. (a) Local Manhattan plot (top) and linkage disequilibrium (LD) heatmap (bottom) surrounding the peak on chromosome 2. The red dashed line indicates the candidate region for the peak. (b) Gene structure of OsGS1;1 and DNA polymorphism in this gene. The gray box represents the promoter region, untranslated regions (UTRs), and introns, and light blue represents coding regions. (c) Comparison between OsGS1;1 haplotypes. (d-e) The plant height and yield per plant of NILHapA and NILHapB in high nitrogen (HN) and low nitrogen (LN) fields. (f-g) The plant height and yield per plant of wild-type (WT) and overexpression (OE) lines in HN and LN fields. Values are mean ± SD (n ≥ 10). Bar = 20 cm. *P< 0.05, **P< 0.01 (Adopted from Liu et al., 2022)

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