Bioscience Methods 2025, Vol.16, No.3, 117-136 http://bioscipublisher.com/index.php/bm 124 Figure 3 Crop diversification in agriculture as manifested by inter-cropping and agro-forestry practices (Adopted from Mihrete and Mihretu, 2025) 4 Practical Application Cases of Agricultural Biotechnology in Industrial Revitalization 4.1 Breeding and genetic resource utilization The application of biological breeding technology is an important means for Pan'an to improve the quality and efficiency of agriculture. Faced with the demand for high-quality varieties in the agricultural product market and the challenges brought by climate change, Pan'an relies on its rich biodiversity to carry out germplasm resource protection and new variety breeding to enhance the competitiveness of characteristic industries. For example, in the tea industry, in response to the problem of insufficient stress resistance of traditional varieties, researchers used molecular marker-assisted breeding technology to select excellent genotypes from 15,000 ancient tea trees for breeding new tea seedlings. At the same time, an ancient tea tree germplasm resource garden was established to collect and preserve the largest number and most complete varieties of ancient tea tree germplasm, laying the foundation for the future cultivation of new tea tree varieties with high fragrance and high stress resistance. In terms of Chinese medicinal materials, Pan'an implemented the "unveiling the list and taking charge" scientific and technological research, and the expert team of Zhejiang Agricultural and Forestry University undertook the key technology research and development tasks for the industrialization of Polygonatum odoratum. The project team collected and evaluated wild Polygonatum sibiricum germplasm, selected excellent strains with short growth cycles and high content of effective ingredients, and developed tissue culture rapid propagation technology and forest-based simulated wild cultivation technology, which greatly improved the supply of Polygonatum sibiricum seedlings and the success rate of planting. Through genetic fingerprinting technology, the DNA identification standard of Pan'an authentic medicinal materials (Rhizoma Corydalis, Atractylodes macrocephala, etc.) was established, effectively protecting the authentic medicinal materials brand (Pan et al., 2024; Yang et al., 2024). In terms of livestock and poultry varieties, Pan'an cooperated with scientific research institutes to carry out seed conservation and breeding of local native pig and chicken breed resources. Using molecular breeding methods, the new Pan'an black pig strain has the characteristics of improved growth performance while retaining meat flavor; Pan'an native chickens have improved egg production through family selection, and the annual egg production of each hen has increased from 80 to more than 120, while the egg quality still maintains the rich flavor of native eggs. Biological breeding is not only reflected in variety improvement, but also includes the construction of a good variety breeding system. Pan'an has established a provincial rice and vegetable seed breeding base, introduced advanced seed processing and preservation technologies, and ensured the large-scale promotion of excellent varieties. Through the exploration of germplasm resources and biotechnology breeding, the speed of variety renewal of Pan'an's main crops and characteristic economic crops has been significantly accelerated, and the yield and quality
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