Bioscience Methods 2025, Vol.16, No.1, 1-10 http://bioscipublisher.com/index.php/bm 7 complicates the breeding process, as it involves multiple genes and complex interactions (Villalobos-López et al., 2022). Additionally, the lack of comprehensive phenotyping tools to assess belowground traits, such as root and tuber development, further hampers the breeding efforts (Harsselaar et al., 2021). The integration of advanced molecular tools and high-throughput phenotyping methods is essential to overcome these limitations and accelerate the development of stress-tolerant cultivars. Figure 3 IbBBX24 overexpression enhances tolerance to salt, drought and oxidative stresses in sweet potato. (a) Responses of IbBBX24-OE, IbBBX24-RNAi and wild-type (WT) sweet potato plants grown for 4 wk on Murashige & Skoog (MS) medium in control conditions (normal) or with 86 mM NaCl or 30% polyethylene glycol 6000 (PEG6000). (b) Responses of IbBBX24-OE, IbBBX24-RNAi and WT sweet potato plants grown hydroponically in half-strength Hoagland solution (normal) or half-strength Hoagland solution containing 86 mM NaCl or 30% PEG6000. (c) Responses of IbBBX24-OE, IbBBX24-RNAi and WT sweet potato plantsgrownintransplantingboxesundercontrolconditions(normal)orsubjectedto200 mMNaCl,droughtor200 μMmethyl viologen (MV). Representative photographs were taken after stress treatment for 4 wk (salt), 8 wk (drought) or 2b wk (MV). Data are shown as means ± SD (n = 3). **, Significant difference from WT at P < 0.01 based on Student’s t-test (Adopted from Zhang et al., 2021)
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