Bioscience Methods 2024, Vol.15, No.3, 91-101 http://bioscipublisher.com/index.php/bm 99 The adoption of molecular diagnostics in veterinary practice has profound implications. It allows for the rapid and specific diagnosis of infectious, neoplastic, and congenital diseases, which is crucial for effective disease management and control. The ability to detect pathogens directly from clinical samples without the need for culture has streamlined the diagnostic process, making it more efficient and less reliant on traditional methods. Moreover, the integration of these advanced diagnostic tools into routine veterinary practice necessitates ongoing education and training for veterinarians to ensure they are well-versed in the use and interpretation of molecular diagnostic tests. This will enable practitioners to make informed decisions regarding disease management and treatment, ultimately improving animal health outcomes. Despite the significant advancements in molecular diagnostics, there is a continuous need for research to further refine and develop these technologies. Future research should focus on enhancing the sensitivity and specificity of diagnostic assays, as well as expanding their applicability to a broader range of pathogens and diseases. Additionally, there is a need to address the challenges associated with quality control and validation of molecular tests to ensure their reliability and accuracy in clinical settings. Collaborative efforts between veterinary researchers, diagnosticians, and practitioners will be essential in driving innovation and improving the overall effectiveness of molecular diagnostics in veterinary medicine. By investing in research and development, the veterinary field can continue to advance its diagnostic capabilities, ultimately leading to better disease control and improved animal health. Acknowledgments Authors are deeply grateful to the two anonymous peer reviewers for their insightful feedback on the manuscript. Conflict of Interest Disclosure Authors affirm that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. Reference Abudayyeh O., and Gootenberg J., 2021, CRISPR diagnostics, Science, 372: 914-915. https://doi.org/10.1126/science.abi9335 Arora N., Chaudhary A., and Prasad A., 2023, Editorial: methods and applications in molecular diagnostics, Frontiers in Molecular Biosciences, 10: 5. https://doi.org/10.3389/fmolb.2023.1239005 Balamurugan V., Venkatesan G., Sen A., Annamalai L., Bhanuprakash V., and Singh R., 2010, Recombinant protein-based viral disease diagnostics in veterinary medicine, Expert Review of Molecular Diagnostics, 10: 731-753. https://doi.org/10.1586/erm.10.61 Belák S., Karlsson O., Blomström A., Berg M., and Granberg F., 2013, New viruses in veterinary medicine, detected by metagenomic approaches, Veterinary Microbiology, 165(1): 95-101. https://doi.org/10.1016/j.vetmic.2013.01.022 Belák S., Thorén P., Leblanc N., and Viljoen G., 2009, Advances in viral disease diagnostic and molecular epidemiological technologies, Expert Review of Molecular Diagnostics, 9: 367-381. https://doi.org/10.1586/erm.09.19 Belák§ S., and Thorén P., 2001, Molecular diagnosis of animal diseases: some experiences over the past decade, Expert Review of Molecular Diagnostics, 1: 434-443. https://doi.org/10.1586/14737159.1.4.434 Bonkobara M., 2016, Recent developments in veterinary diagnostics: current status and future potential, Veterinary Journal, 215(1-2): 16-19. https://doi.org/10.1016/j.tvjl.2016.08.010 Cai H., Caswell J., and Prescott J., 2014, Nonculture molecular techniques for diagnosis of bacterial disease in animals, Veterinary Pathology, 51: 341-350. https://doi.org/10.1177/0300985813511132 Choi J., Tang R., Wang S., Abas W., Pingguan-Murphy B., and Xu F., 2015, Paper-based sample-to-answer molecular diagnostic platform for point-of-care diagnostics, Biosensors & Bioelectronics, 74: 427-439. https://doi.org/10.1016/j.bios.2015.06.065 Daniels J., 2013, Molecular diagnostics for infectious disease in small animal medicine: an overview from the laboratory, The Veterinary Clinics of North America, Small Animal Practice, 43: 1373-1384. https://doi.org/10.1016/j.cvsm.2013.07.006 Fortina P., Surrey S., and Kricka L., 2002, Molecular diagnostics: hurdles for clinical implementation, Trends in Molecular Medicine, 8(6): 264-266. https://doi.org/10.1016/S1471-4914(02)02331-6
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