Bioscience Evidence 2024, Vol.14, No.5, 218-226 http://bioscipublisher.com/index.php/be 218 Research Article Open Access Molecular Mechanisms of the Rice HAM Domain Gene OsHIPP16 in Ovule Development Yumin Huang School of Life Science, Xiamen University, Xiamen, 361102, Fujian, China Corresponding email: hym@xmu.edu.cn Bioscience Evidence, 2024, Vol.14, No.5 doi: 10.5376/be.2024.14.0023 Received: 01 Aug., 2024 Accepted: 05 Sep., 2024 Published: 22 Sep., 2024 Copyright © 2024 Huang, This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: Huang Y.M., 2024, Molecular mechanisms of the rice HAM domain gene OsHIPP16 in ovule development, Bioscience Evidence, 14(5): 218-226 (doi: 10.5376/be.2024.14.0023) Abstract Transcriptomic analyses have identified many differentially expressed genes (DEGs) at different stages of ovule development, suggesting complex regulatory networks are involved. Studies have shown that genes related to cell component biogenesis, membrane-binding organelles, and reproductive regulation are highly expressed during ovule development, and the role of sporophyte tissue in female gametophyte development has been confirmed by gene expression profiles. Relevant studies have also discussed the interaction of OsHIPP16 with other proteins and its potential regulatory functions in ovule development. Providing new insights into its molecular mechanisms, OsHIPP16 plays a crucial role in rice ovule development through complex molecular and cellular events. The interaction of this gene with various regulatory networks and its expression patterns suggest that it contributes significantly to the formation of female gametophytes. These findings enhance researchers' understanding of the genetic and molecular basis of rice ovule development and provide potential applications for improving rice fertility and crop yield. The aim of this study was to elucidate the molecular mechanism of rice HAM domain gene OsHIPP16 in ovule development and to understand its role in female gametophyte formation and function in rice (Oryza sativa). Keywords OsHIPP16; Ovule development; Rice; Female gametophyte; Gene expression 1 Introduction Ovule development in rice (Oryza sativa) is a critical process that significantly impacts the plant's reproductive success and seed yield. The ovule, which eventually develops into a seed post-fertilization, is essential for the continuation of the species and for agricultural productivity. Understanding the molecular events associated with ovule development can provide insights into improving rice fertility and yield. Recent studies have utilized RNA sequencing to profile gene expression during various stages of ovule development, identifying numerous differentially expressed genes (DEGs) that play crucial roles in this process. These genes are involved in cellular component biogenesis, membrane-bounded organelles, and reproductive regulation, highlighting the complexity of molecular and cellular events during ovule development (Wu et al., 2015). Additionally, the regulatory networks involving transcription factors, plant hormones, and epigenetic modifications have been shown to be critical in the formation and fertility of the female gametophyte (Yang et al., 2016). HAM domain genes are a group of genes that encode proteins with a highly conserved domain known as the HAM (Histidine Acidic Motif) domain. These genes are involved in various developmental processes in plants, including ovule development. The HAM domain proteins are known to interact with other proteins and play roles in transcriptional regulation, signal transduction, and cellular differentiation. In rice, the expression of microRNAs (miRNAs) during ovule development has been shown to regulate the interaction between sporophytic tissue and the female gametophyte, indicating a complex regulatory network involving HAM domain genes (Wu et al., 2017). The identification and functional analysis of these genes can provide valuable insights into the molecular mechanisms underlying ovule development and fertility in rice. By integrating data from various studies, this study aims to elucidate the role of OsHIPP16 in the regulation of ovule development, identify key regulatory networks and pathways, and provide a comprehensive understanding
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