International Journal of Aquaculture, 2025, Vol.15, No.5, 229-239 http://www.aquapublisher.com/index.php/ija 234 These differential sites may be related to the traits of artificial breeding, suggesting that targeted allelic modifications have occurred in Chinese shrimp. Figure 1 BCPAP PTC cell lines were transfected with METTL3-overexpression (METTL3) plasmids and siRNA targeting STEAP2 (si-STEAP2) as indicated (Adopted from Zhu et al., 2022) Image caption: A, B Effect of METTL3/STEAP2 axis on the expression of Hedgehog signaling pathway-related proteins (Smo, Gli1, and PTCH1) and EMT markers (E-cadherin, N-cadherin, snail, vimentin, and β-catenin) in PTC cells were quantified by western blot assay. C The graphic illustration of METTL3-mediated m6A modification modulating malignant phenotype of PTC through enhancing STEAP2 expression and then restraining Hedgehog signaling pathway and EMT. All data are presented as the mean ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 (Adopted from Zhu et al., 2022) 6.2 GWAS reveals the molecular mechanism of key growth traits of Chinese shrimp Compared with vannabinoid prawns, there are fewer studies on whole genome association analysis (GWAS) of Chinese prawns. The genetic background differences in Chinese prawns may lead to different growth regulation mechanisms from those of vannabinoid prawns. Chinese prawns are more tolerant of low temperatures but grow slowly, which may mean that their growth may be controlled by some genes related to energy metabolism and stress response. According to population comparison research, some of the differences between Chinese shrimp breeding population and wild population are located on immune and stress-resistant genes. This suggests that in order to improve survival rate and disease resistance during the breeding process, it may indirectly affect growth-related pathways-faster-growing families are often families with better stress resistance, because improving health status is conducive to growth. Further, drawing on the discovery of vannabinoid shrimp, we can pay attention to some common candidate genes. For example, the insulin-like peptide ILP pathway is highly conserved in crustaceans, and similar growth hormones are likely to exist in Chinese shrimp. If differentially expressed genes of fast-growing families can be screened through transcriptome sequencing, ILP, its receptors, downstream signals, etc. are potential targets of concern (Su et al., 2024). In recent years, studies have conducted transcriptome and metabolomic analyses on Chinese shrimp, trying to reveal the molecular characteristics of rapidly growing individuals. The results show that fast-growing Chinese shrimps have a series of metabolic enzyme genes upregulated in muscle tissue, such as
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