IJA_2025v15n4

International Journal of Aquaculture, 2025, Vol.15, No.4, 197-207 http://www.aquapublisher.com/index.php/ija 206 methylation maps, histone modification sites, and highly expressed ncRNA catalogs. Researchers can obtain the epigenetic status of target genes in different environments and developmental stages on these platforms in one-stop, thereby proposing more targeted hypotheses and designing experimental verification. Acknowledgments The authors would like to thank all the teachers and colleagues who provided guidance and assistance to Cuixi Biotechnology Research Institute during this research, and thank the peer review for their revision suggestions. Conflict of Interest Disclosure The authors confirm that the study was conducted without any commercial or financial relationships and could be interpreted as a potential conflict of interest. References Bonin C., Van Wijnen A., and Lewallen E., 2019, MicroRNA applications in marine biology, Current Molecular Biology Reports, 5: 167-175. https://doi.org/10.1007/s40610-019-00124-w Dellong A., Fallet M., Stenger P., Chaparro C., Vidal-Dupiol J., Clément J., Cosseau C., and Grunau C., 2024, Benchmarking of chromatin immunoprecipitation (ChIP-Seq) methods in the Pacific oyster Magallana gigas, BioRxiv, 2024: 12. https://doi.org/10.1101/2024.12.28.630603 Fellous A., Franc L., Jouaux A., Goux D., Favrel P., and Rivière G., 2019, Histone methylation participates in gene expression control during the early development of the pacific oyster Crassostrea gigas, Genes, 10(9): 695. https://doi.org/10.3390/genes10090695 Gawra J., Valdivieso A., Roux F., Laporte M., De Lorgeril J., Gueguen Y., Saccas M., Escoubas J., Montagnani C., Destoumieux-Garzόn D., Lagarde F., Leroy M., Haffner P., Petton B., Cosseau C., Morga B., Dégremont L., Mitta G., Grunau C., and Vidal-Dupiol J., 2023, Epigenetic variations are more substantial than genetic variations in rapid adaptation of oyster to Pacific oyster mortality syndrome, Science Advances, 9(36): eadh8990. https://doi.org/10.1126/sciadv.adh8990 Hong W.Y., and Mai R.D., 2025, Global biogeographic patterns and genetic connectivity of oyster populations, International Journal of Marine Science, 15(1): 15-27. https://doi.org/10.5376/ijms.2025.15.0002 Johnson K., and Kelly M., 2020, Population epigenetic divergence exceeds genetic divergence in the Eastern oyster Crassostrea virginica in the Northern gulf of Mexico, Evolutionary Applications, 13: 945-959. https://doi.org/10.1111/eva.12912 Kundu M., and Basu J., 2021, The role of microRNAs and long non-coding RNAs in the regulation of the immune response to Mycobacteriumtuberculosis infection, Frontiers in Immunology, 12: 687962. https://doi.org/10.3389/fimmu.2021.687962 Li C., Wang H., and Guo X., 2022, Regulation of the cell cycle apoptosis and proline accumulation plays an important role in the stress response of the eastern oyster Crassostrea Virginica, Frontiers in Marine Science, 9: 921877. https://doi.org/10.3389/fmars.2022.921877 Li J., He Y., Yang B., Mokrani A., Li Y., Tan C., Li Q., and Liu S., 2024, Whole-genome DNA methylation profiling revealed epigenetic regulation of NF-κB signaling pathway involved in response to Vibrio alginolyticus infection in the Pacific oyster Crassostrea gigas, Fish and Shellfish Immunology, 151: 109705. https://doi.org/10.1016/j.fsi.2024.109705 Li X., Bai Y., Xu C., Liu S., Yu H., Kong L., Du S., and Li Q., 2024, OysterDB: a genome database for ostreidae, Marine Biotechnology, 26(4): 827-834. https://doi.org/10.1007/s10126-024-10327-7 Li Y., Teng W., Xu C., Yu H., Kong L., Liu S., and Li Q., 2022, Intergenerational transfer and sex differences of DNA methylation patterns in the Pacific oyster (Crassostrea gigas), BioRxiv, 2022: 02. https://doi.org/10.1101/2022.02.22.481396 Morita S., Horii T., and Hatada I., 2024, Optimized protocol for the regulation of DNA methylation and gene expression using modified dCas9-suntag platforms, Methods in Molecular Biology, 2842: 155-165. https://doi.org/10.1007/978-1-0716-4051-7_7 Nahand J., Karimzadeh M., Nezamnia M., Fatemipour M., Khatami A., Jamshidi S., Moghoofei M., Taghizadieh M., Hajighadimi S., Shafiee A., Sadeghian M., Bokharaei-Salim F., and Mirzaei H., 2019, The role of miR‐146a in viral infection, IUBMB Life, 72: 343-360. https://doi.org/10.1002/iub.2222 Qian J., and Liu S.X., 2024, CRISPR/dCas9-tet1-mediated DNA methylation editing, Bio-Protocol, 14(8): e4976-e4976. https://doi.org/10.21769/BioProtoc.4976 Rivière G., Wu G., Fellous A., Goux D., Sourdaine P., and Favrel P., 2013, DNA methylation is crucial for the early development in the oyster C. gigas,Marine Biotechnology, 15: 739-753. https://doi.org/10.1007/s10126-013-9523-2

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