International Journal of Aquaculture, 2024, Vol.14, No.1, 37-39 http://www.aquapublisher.com/index.php/ija 37 Scientific Commentary Open Access Redefining Intercellular Signaling: Trafficking Mechanism of The Wnt5b-Ror2 Complex in Zebrafish Sarah McGrew International Journal of Aquaculture, Aqua Publisher, Richmond, BC, V7A 4Z5, Canada Corresponding email: sarah.mcgrew@sophiapublisher.com International Journal of Aquaculture, 2024, Vol.14, No.1 doi: 10.5376/ija.2024.14.0005 Received: 30 Dec., 2023 Accepted: 12 Feb., 2024 Published: 22 Feb., 2024 Copyright © 2024 McGrew, This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Preferred citation for this article: McGrew S., 2024, Redefining intercellular signaling: trafficking mechanism of the Wnt5b-Ror2 complex in zebrafish, International Journal of Aquaculture, 14(1): 37-39 (doi: 10.5376/ija.2024.14.0005) The article titled "Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish" was written by Steffen Scholpp and other research teams and was published in "Nature" magazine on December 20, 2023. The research team includes a number of biologists and oceanographic experts who are dedicated to uncovering cell signaling mechanisms.This study explores the trafficking mechanism of Wnt5b and Ror2 complexes in zebrafish embryos. Wnt5b is an important ligand in the Wnt signaling pathway, and Ror2 is its receptor. Both of them play a role in cell polarity and migration. The study found that Wnt5b forms a complex with Ror2 in producer cells and is transported from the producer cell to the recipient cell through cell protrusions (called protrusions). Cell protrusions are elongated cell membrane extensions that serve as signaling "highways" to carry signaling molecules from one cell to another. The research team observed Wnt5b and Ror2 in detail through high-resolution imaging techniques. The results indicate that the Wnt5b-Ror2 complex remains intact during transport and enters the receiving cell via dynein-dependent endocytosis, activating the Wnt-PCP signaling pathway. This mechanism not only enhances the efficiency of signal transmission, but may also influence cell behavior during embryonic development. This study provides new insights into the precise transport of signaling molecules in embryonic development and helps to re-evaluate traditional modes of cell-to-cell communication, which has important applications in aquaculture. 1 A Multi-Technology Approach Using Zebrafish Embryos and CRISPR-Cas9 The study is exquisitely designed and combines a variety of advanced experimental techniques to comprehensively reveal the cell protrusion-mediated transport mechanism of the Wnt5b-Ror2 complex. The research team used high-resolution imaging technology and the application of zebrafish embryos and fibroblast cell models to make the research results have broad biological significance. The use of CRISPR-Cas9 gene editing technology further verified the functions of Wnt5b and Ror2 in the signaling process. This multi-technology, multi-model comprehensive research design ensures the reliability of data and the accuracy of results, and provides a new perspective for revealing intercellular signaling. 2 High-Resolution Imaging Reveals Wnt5b-Ror2 Complex Transport and Signaling in Zebrafish Embryos This article uses high-resolution imaging technology to analyze the transport process of Wnt5b and Ror2 complexes on cell protrusions. The study showed that Wnt5b and Ror2 co-localized on cell processes of zebrafish fibroblasts and ectodermal cells, and their presence was confirmed by fluorescent labeling and antibody staining. Live-cell imaging showed that the Wnt5b-Ror2 complex remained intact during transport, entering the receiving cell via dynein-dependent endocytosis. FLIM-FRET and FCCS analyzes further confirmed the stability of the interaction between Wnt5b and Ror2 during transport, measuring the distance and interaction force between them. Experimental results also show that cell protrusion-mediated Wnt5b-Ror2 transmission activates the Wnt-PCP signaling pathway in zebrafish embryos, affecting cell migration and tissue polarity during embryonic development.
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