Animal Molecular Breeding 2024, Vol.14, No.1, 72-81 http://animalscipublisher.com/index.php/amb 76 Figure 1 Different coat colors exhibited by different DNA methylation in Pomeranian dogs As Pomeranians grow, their DNA methylation profiles also show dynamic changes. Early research mainly focused on Pomeranian individuals at different developmental stages to explore the changes in DNA methylation during the life cycle. These studies found that during the Pomeranian embryonic development stage, the DNA methylation levels of certain loci showed specific spatiotemporal patterns, which were closely related to the regulation of gene expression during embryonic development. As Pomeranians grow, DNA methylation also shows clear differences between adolescence and adulthood. This may be related to Pomeranian's adaptation to the environment and lifestyle at different developmental stages, and also provides a basis for subsequent exploration of the biological function of DNA methylation in the Pomeranian's life cycle. 3.2 Technical means and methods In the study of DNA methylation in Pomeranians, methylation-sensitive PCR technology is widely used. This technology selectively amplifies DNA fragments with specific methylation status by exploiting the differences between DNA methylation and non-methylation. Researchers can quantitatively assess the methylation levels of specific loci in the Pomeranian genome by analyzing the quantity and quality of PCR products. Methylation-sensitive PCR not only has high sensitivity, but also can perform high-throughput analysis in larger-scale samples, providing strong technical support for large-scale DNA methylation surveys. In recent years, with the rapid development of biotechnology, methylomics has become an important means to study DNA methylation in Pomeranians. Methylomics combines high-throughput sequencing technology and bioinformatics analysis methods to comprehensively and efficiently reveal DNA methylation information in the Pomeranian genome. Through methylomics, researchers can more comprehensively understand the spatial distribution, specific patterns of DNA methylation in Pomeranians, and the underlying mechanisms related to genetic traits. Methylomics not only provides a high-resolution map of the DNA methylation status of the entire genome, but can also reveal the methylation heterogeneity in different gene regions, providing new insights into the epigenetic regulatory mechanism of Pomeranians. perspective. At the same time, by integrating methylome data with other
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