Animal Molecular Breeding 2024, Vol.14, No.1, 19-26 http://animalscipublisher.com/index.php/amb 24 A typical application of this experimental approach is to gain insights into genes related to social behavior by recording cats' social behaviors, such as intimate licking and sharing meals. At the same time, by analyzing the dynamic changes in gene expression when these behaviors occur, researchers can discover the spatiotemporal regulation patterns of specific genes in social interactions, providing important clues for analyzing their role in complex social networks. 3.2.2 Effects of genetic mutations on cat physiological processes In order to simulate the variation in the cat genome, introducing gene mutations has become an effective method (Figure 3). By introducing mutations in specific genes into the cat genome, researchers can observe the direct effects of these variations on physiological processes. For example, by simulating mutations in a certain behavioral-related gene, researchers can observe whether cats' social behavior is inhibited and whether they exhibit abnormal behavior patterns. Figure 3 Introduction of mutated genes (Nebert, 1999) The advantage of this experimental design is that it can precisely control the variation of genes, making it more consistent with the mutation situation in real organisms. By comparing the behavioral and physiological differences between normal cats and genetically mutated cats, this study can provide an in-depth understanding of the precise regulation of key genes on cat physiological processes, and then reveal the exact role of these genes in cat behavior and physiological functions. This series of experimental methods will provide this study with a more in-depth and comprehensive analysis of key gene functions, and provide rich empirical data for the study of cat behavior and physiological characteristics. This is not only of great value for research in the fields of cat behavior and physiology, but also provides useful insights into the relationship between human genes and behavior. 4 Cat Gene Interaction Networks 4.1 Protein interaction network Cat genome encode proteins that form an intricate, coordinated network of interactions. Through highly sophisticated protein mass spectrometry technology and bioinformatics analysis, this study successfully revealed the direct or indirect interactions between genes and proteins in this huge network. In the cat protein interaction network, some genetic proteins were found to play key roles as central nodes. These central nodes connect multiple interconnected genes and proteins, forming a functionally highly integrated network hub. This study will conduct an in-depth study of the functions of these central nodes and explore their specific roles in regulating cat physiology and behavior. The functions of these central nodes may cover multiple levels, including but not limited to regulating cell signaling, participating in gene expression regulation, and affecting metabolic pathways (Armingol et al., 2021). This study will reveal the molecular mechanisms of these central nodes in the cat genome through further biological experiments and bioinformatics analysis, providing strong support for a deep understanding of the biological functions of cat genes.
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